Document Detail


Accumulation of pyroglutamic acid (5-oxoproline) in homocystinuria.
MedLine Citation:
PMID:  7134819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A 12-year-old boy with homocystinuria due to a cystathionine synthetase defect was shown to accumulate large amounts of pyroglutamic acid (pyroglu). In four different urine samples the concentration ranged between 4.1 and 9.7 mol/mol of creatinine. The pyroglu concentration in plasma was 1.6 mmol/l, and in erythrocytes 750 nmol/g cells. Normally only trace amounts are present. The pyroglu levels normalized during treatment with pyridoxine and folic acid. Accumulation of pyroglu has not previously been described in homocystinuria. Homocysteine can replace cysteine as a substrate for gamma-glutamylcysteine synthetase, an enzyme involved in the biosynthesis of glutathione, giving gamma-glutamylhomocysteine. This dipeptide is efficiently split by gamma-glutamylcyclotransferase to pyroglu and homocysteine. This reaction sequence most likely explains the accumulation of pyroglu in our patient. Ten other homocystinuria patients, who all had lower plasma levels of homocysteine, did not accumulate pyroglu. Our patient might either be a new variant, or more likely the pyroglu formation occurs only high levels of homocysteine.
Authors:
O Stokke; S Marstein; E Jellum; S O Lie
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  42     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  1982 Jun 
Date Detail:
Created Date:  1982-12-18     Completed Date:  1982-12-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  361-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / blood
Animals
Child
Cystathionine beta-Synthase / metabolism
Fibroblasts / enzymology
Glutathione / blood
Guinea Pigs
Homocystinuria / urine*
Humans
Male
Methionine / metabolism
Pyroglutamate Hydrolase / metabolism
Pyrrolidinones / urine*
Pyrrolidonecarboxylic Acid / urine*
Tissue Extracts / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Pyrrolidinones; 0/Tissue Extracts; 63-68-3/Methionine; 70-18-8/Glutathione; 98-79-3/Pyrrolidonecarboxylic Acid; EC 3.5.2.-/Pyroglutamate Hydrolase; EC 4.2.1.22/Cystathionine beta-Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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