Document Detail


Accumulation of non-superoxide anion reactive oxygen species mediates nitrogen-limited alcoholic fermentation by Saccharomyces cerevisiae.
MedLine Citation:
PMID:  20952643     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Throughout alcoholic fermentation, nitrogen depletion is one of the most important environmental stresses that can negatively affect the yeast metabolic activity and ultimately leads to fermentation arrest. Thus, the identification of the underlying effects and biomarkers of nitrogen limitation is valuable for controlling, and therefore optimizing, alcoholic fermentation. In this study, reactive oxygen species (ROS), plasma membrane integrity, and cell cycle were evaluated in a wine strain of Saccharomyces cerevisiae during alcoholic fermentation in nitrogen-limiting medium under anaerobic conditions. The results indicated that nitrogen limitation leads to an increase in ROS and that the superoxide anion is a minor component of the ROS, but there is increased activity of both Sod2p and Cta1p. Associated with these effects was a decrease in plasma membrane integrity and a persistent cell cycle arrest at G(0)/G(1) phases. Moreover, under these conditions it appears that autophagy, evaluated by ATG8 expression, is induced, suggesting that this mechanism is essential for cell survival but does not prevent the cell cycle arrest observed in slow fermentation. Conversely, nitrogen refeeding allowed cells to reenter cell cycle by decreasing ROS generation and autophagy. Altogether, the results provide new insights on the understanding of wine fermentations under nitrogen-limiting conditions and further indicate that ROS accumulation, evaluated by the MitoTracker Red dye CM-H(2)XRos, and plasma membrane integrity could be useful as predictive markers of fermentation problems.
Authors:
Ana Mendes-Ferreira; Belém Sampaio-Marques; Catarina Barbosa; Fernando Rodrigues; Vítor Costa; Arlete Mendes-Faia; Paula Ludovico; Cecília Leão
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-15
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-03     Completed Date:  2011-03-14     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7918-24     Citation Subset:  IM    
Affiliation:
Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Alcohols / metabolism*
Autophagy / drug effects
Cell Cycle / drug effects
Cell Membrane / drug effects,  physiology
Culture Media / chemistry
Fermentation
Microbial Viability / drug effects
Nitrogen / metabolism*
Reactive Oxygen Species / toxicity*
Saccharomyces cerevisiae / drug effects*,  growth & development,  metabolism*,  physiology
Superoxides / analysis*
Chemical
Reg. No./Substance:
0/Alcohols; 0/Culture Media; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 7727-37-9/Nitrogen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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