Document Detail


Accumulation of nitrotyrosine correlates with endothelial NO synthase in pulmonary resistance arteries during chronic hypoxia in the rat.
MedLine Citation:
PMID:  10930354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitrotyrosine and eNOS were detected immunocytochemically using specific antibodies in paraffin sections of lung from rats subjected to hypoxia for 2, 7, or 14 days. The staining intensity for eNOS was enhanced in the endothelium of both resistance and conduit pulmonary arteries at 2 days. Staining intensity for eNOS remained elevated at 7 and 14 days in conduit arteries, whereas it progressively increased further in resistance arteries. Nitrotyrosine staining was elevated to a similar degree in endothelium and adjacent vascular smooth muscle. In resistance pulmonary arteries, there was a progressive increase in nitrotyrosine, which matched the increase in eNOS. In conduit pulmonary arteries, nitrotyrosine increased only after 14 days of hypoxia. The results suggest that in chronic hypoxia the up-regulation of eNOS leads to the formation of peroxynitrite which has access to both endothelium and vascular smooth muscle.
Authors:
A T Demiryürek; M R Karamsetty; A R McPhaden; R M Wadsworth; K A Kane; M R MacLean
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pulmonary pharmacology & therapeutics     Volume:  13     ISSN:  1094-5539     ISO Abbreviation:  Pulm Pharmacol Ther     Publication Date:  2000  
Date Detail:
Created Date:  2000-10-12     Completed Date:  2000-10-12     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9715279     Medline TA:  Pulm Pharmacol Ther     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  157-65     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
Department of Physiology & Pharmacology, University of Strathclyde, Glasgow, G4 0NR, UK.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Anoxia / complications,  metabolism*
Arteries / metabolism,  pathology
Endothelium / metabolism
Hypertrophy, Right Ventricular / etiology
Immunohistochemistry
Lung / blood supply*,  metabolism,  pathology
Nitric Oxide Synthase / metabolism*
Rats
Tyrosine / analogs & derivatives*,  metabolism
Vascular Resistance
Chemical
Reg. No./Substance:
0/Actins; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC 1.14.13.39/Nitric Oxide Synthase

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