Document Detail

Accumulation of (n-9)-eicosatrienoic and docosatrienoic acids in human fibroblast phospholipids.
MedLine Citation:
PMID:  3185126     Owner:  NLM     Status:  MEDLINE    
An essential fatty acid (EFA) deficiency-like profile of fatty acids has been observed in HF-1 human skin fibroblasts cultured at clonal densities in MCDB 110 and 0.4% fetal bovine serum (FBS). The profile was characterized by an accumulation of 16:1n-7, 18:1n-9, 20:3n-9 and 22:3n-9, a reduction of n-6 fatty acids and a reduction in total polyunsaturated fatty acids (PUFA). The fatty acid composition of sphingomyelin (SPH), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylethanolamine (PE) was determined and, except for SPH, each displayed an EFA deficiency-like profile. The triene to tetraene ratio (20:3n-9/20:4n-6) ranged from 5.3 in PI to 0.9 in PE. In addition, the highest percentage of 20:3n-9 was present in the PI and the highest percentage of 22:3n-9, in PE. Other human fibroblasts (normal, transformed and at different population doubling number levels [PDL]) were grown under the same conditions and were found to display triene to tetraene ratios (20:3n-9/20:4n-6) in total cellular lipids ranging from 0.7 to 4.5. The accumulation of 20:3n-9 and 22:3n-9 is due primarily to the existence of a basal nutrient medium (MCDB 110) that allows for the rapid clonal growth of human fibroblasts at reduced serum levels (0.4%). This culture procedure can be exploited to further elucidate various aspects of lipid metabolism in human fibroblasts.
S Karmiol; W J Bettger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  23     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-12-22     Completed Date:  1988-12-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  891-8     Citation Subset:  IM    
Dept. of Nutritional Sciences, College of Biological Science, University of Guelph, Ontario.
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MeSH Terms
8,11,14-Eicosatrienoic Acid / metabolism*
Cell Survival
Cells, Cultured
Fatty Acids, Essential / deficiency
Fatty Acids, Unsaturated / metabolism*
Fibroblasts / metabolism*
Phospholipids / metabolism*
Reg. No./Substance:
0/Fatty Acids, Essential; 0/Fatty Acids, Unsaturated; 0/Phospholipids; 7324-41-6/8,11,14-Eicosatrienoic Acid

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