| Accumulation of mutants in "aging" bacterial colonies is due to growth under selection, not stress-induced mutagenesis. | |
| | |
MedLine Citation:
|
PMID: 18701713 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Several bacterial systems show behavior interpreted as evidence for stress-induced mutagenesis (adaptive mutation), a postulated process by which nongrowing cells temporarily increase their general mutation rate. Theoretical considerations suggest that periodic stress-induced general mutagenesis would not be advantageous in the long term, due to the high cost of deleterious mutations. Alternative explanations have been tested for very few of the systems used as evidence for stress-induced mutation. In one prominent system, mutants resistant to rifampicin (Rif(R); rpoB; RNA polymerase) accumulate in cell populations that "age" on solid medium with little net growth. Mutant accumulation was initially attributed to stress-induced general mutagenesis in nongrowing cells. Evidence is presented that these Rif(R) mutants accumulate because they grow faster than parent cells during the aging period. Direct tests revealed no increase in the frequency of other mutant types during the aging period. |
| | |
Authors:
|
Marie Wrande; John R Roth; Diarmaid Hughes |
Related Documents
:
|
158683 - Effect of cell density and senescence of wi-38 cells on cytochemically demonstrable pho... 15857403 - Misregulation of poly(adp-ribose) polymerase-1 activity and cell type-specific loss of ... 207533 - Conditions for maximal synthesis of cyclic amp by mouse macrophages in response to beta... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-08-13 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 105 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2008 Aug |
Date Detail:
|
Created Date: 2008-08-20 Completed Date: 2008-09-16 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 11863-8 Citation Subset: IM |
Affiliation:
|
Department of Cell and Molecular Biology, Box 596, The Biomedical Center, Uppsala University, S-75124 Uppsala, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adaptation, Biological Colony Count, Microbial Drug Resistance, Bacterial / drug effects Escherichia coli / drug effects, genetics*, growth & development* Mutagenesis* Mutation / genetics Rifampin / pharmacology Salmonella enterica / drug effects, genetics*, growth & development* Selection, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
|
GM27068/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
13292-46-1/Rifampin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transactivation of EGF receptor and ErbB2 protects intestinal epithelial cells from TNF-induced apop...
Next Document: The CD83 reporter mouse elucidates the activity of the CD83 promoter in B, T, and dendritic cell pop...