Document Detail


Accumulation of multiple-repeat starch-binding domains (SBD2-SBD5) does not reduce amylose content of potato starch granules.
MedLine Citation:
PMID:  17039369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigates whether it is possible to produce an amylose-free potato starch by displacing the amylose enzyme, granule-bound starch synthase I (GBSSI), from the starch granule by engineered, high-affinity, multiple-repeat family 20 starch-binding domains (SBD2, SBD3, SBD4, and SBD5). The constructs were introduced in the amylose-containing potato cultivar (cv. Kardal), and the starches of the resulting transformants were compared with those of SBD2-expressing amylose-free (amf) potato clones. It is shown that a correctly sized protein accumulated in the starch granules of the various transformants. The amount of SBD accumulated in starch increased progressively from SBD to SBD3; however, it seemed as if less SBD4 and SBD5 was accumulated. A reduction in amylose content was not achieved in any of the transformants. However, it is shown that SBDn expression can affect physical processes underlying granule assembly, in both genetic potato backgrounds, without altering the primary structure of the constituent starch polymers and the granule melting temperature. Granule size distribution of the starches obtained from transgenic Kardal plants were similar to those from untransformed controls, irrespective of the amount of SBDn accumulated. In the amf background, granule size is severely affected. In both the Kardal and amf background, apparently normal oval-shaped starch granules were composed of multiple smaller ones, as evidenced from the many "Maltese crosses" within these granules. The results are discussed in terms of different binding modes of SBD.
Authors:
Farhad Nazarian Firouzabadi; Jean-Paul Vincken; Qin Ji; Luc C J M Suurs; Alain Buléon; Richard G F Visser
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta     Volume:  225     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  919-33     Citation Subset:  IM    
Affiliation:
Graduate School Experimental Plant Sciences, Laboratory of Plant Breeding, Wageningen University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Amylose / chemistry,  metabolism*,  ultrastructure
Cytoplasmic Granules / metabolism*,  ultrastructure
Gene Expression
Genes, Plant
Particle Size
Plant Tubers / metabolism*
Solanum tuberosum / metabolism*
Starch Synthase / metabolism*
Tandem Repeat Sequences
Transformation, Genetic
Chemical
Reg. No./Substance:
9005-82-7/Amylose; EC 2.4.1.-/granule-bound starch synthase I; EC 2.4.1.21/Starch Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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