Document Detail


Accumulation of macrophage-like cells expressing NG2 proteoglycan and Iba1 in ischemic core of rat brain after transient middle cerebral artery occlusion.
MedLine Citation:
PMID:  17565360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although neurons and glia inevitably undergo degeneration in the core of ischemic lesions, many cells, particularly immune cells, infiltrate the core and survive in it. Such infiltrating cells may play certain roles in the regeneration and repair of damaged brain tissues. In this study, we characterized macrophage-like cells that accumulated in the ischemic core of a rat brain whose right middle cerebral artery was transiently occluded for 90 mins. Many of the accumulated macrophage-like cells expressed Iba1, a marker of macrophages/microglia, as well as NG2 chondroitin sulfate proteoglycan (NG2), which has been recognized as a marker of oligodendrocyte progenitor cells. Such macrophage-like cells were termed BINCs (brain Iba1(+)/NG2(+) cells) to distinguish them from NG2(-)/Iba1(+) or NG2(+)/Iba1(-) cells that were also present in the perilesion and the contralateral hemisphere. Electron microscopy showed the localization of NG2 along the plasma membrane of cells that had many phagosomes and irregular-shaped or reniform heterochromatin-rich nuclei, which are characteristics of monocytes/macrophages. Brain Iba1(+)/NG2(+) cells were highly proliferative and their number peaked at 7 days post-reperfusion. An immunoblot analysis of NG2 revealed the presence of two NG2s: one expressed by BINCs with a molecular weight of 300 kDa, and the other found in the contralateral hemisphere with a molecular weight of 290 kDa. Taken the various functions of NG2, BINCs may be involved in not only phagocytosis of degenerated cells but also the healing and regeneration of lesion cores.
Authors:
Hiroaki Matsumoto; Yoshiaki Kumon; Hideaki Watanabe; Takanori Ohnishi; Masachika Shudou; Miao Chuai; Yoshinori Imai; Hisaaki Takahashi; Junya Tanaka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-13
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  28     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-21     Completed Date:  2008-03-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  149-63     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Graduate School of Medicine, Ehime University, Toon, Ehime, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / biosynthesis*
Antigens, Differentiation / biosynthesis
Calcium-Binding Proteins / biosynthesis*
Cell Membrane / metabolism,  ultrastructure
Gene Expression Regulation*
Heterochromatin / metabolism,  ultrastructure
Infarction, Middle Cerebral Artery / metabolism*,  pathology
Macrophages / metabolism*,  ultrastructure
Male
Microglia / metabolism,  ultrastructure
Monocytes / metabolism,  ultrastructure
Oligodendroglia / metabolism,  ultrastructure
Phagosomes / metabolism,  ultrastructure
Proteoglycans / biosynthesis*
Rats
Rats, Wistar
Regeneration*
Stem Cells / metabolism,  ultrastructure
Time Factors
Chemical
Reg. No./Substance:
0/Aif1 protein, rat; 0/Antigens; 0/Antigens, Differentiation; 0/Calcium-Binding Proteins; 0/Heterochromatin; 0/Proteoglycans; 0/chondroitin sulfate proteoglycan 4

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