Document Detail

Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica.
MedLine Citation:
PMID:  22752918     Owner:  NLM     Status:  MEDLINE    
Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic sequences, including glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate acyltransferase, lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, and phospholipid:diacylglycerol acyltransferase, can be useful for manipulating metabolic pathways for biofuel production. In this study, we found this approach indeed increased the storage lipid content of C. minutissima UTEX 2219 up to 2-fold over that of wild type. Thus, we conclude this approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity.
Hsin-Ju Hsieh; Chia-Hung Su; Liang-Jung Chien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-30
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  50     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-10-29     Revised Date:  2012-10-31    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  526-34     Citation Subset:  IM    
Material and Chemical Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu Rd., Sec. 2, Hsinchu, 30011, Taiwan.
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MeSH Terms
Biosynthetic Pathways / genetics*
Chlorella / genetics,  metabolism*
Cloning, Molecular
Lipid Metabolism*
Metabolic Engineering*
Recombinant Proteins / genetics,  metabolism
Saccharomyces cerevisiae / enzymology*,  genetics
Triglycerides / biosynthesis*
Yarrowia / enzymology*,  genetics
Reg. No./Substance:
0/Biofuels; 0/Recombinant Proteins; 0/Triglycerides
Erratum In:
J Microbiol. 2012 Aug;50(4):716

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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