| Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica. | |
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MedLine Citation:
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PMID: 22752918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic sequences, including glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate acyltransferase, lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, and phospholipid:diacylglycerol acyltransferase, can be useful for manipulating metabolic pathways for biofuel production. In this study, we found this approach indeed increased the storage lipid content of C. minutissima UTEX 2219 up to 2-fold over that of wild type. Thus, we conclude this approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity. |
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Authors:
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Hsin-Ju Hsieh; Chia-Hung Su; Liang-Jung Chien |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-06-30 |
Journal Detail:
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Title: Journal of microbiology (Seoul, Korea) Volume: 50 ISSN: 1976-3794 ISO Abbreviation: J. Microbiol. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-07-03 Completed Date: 2012-10-29 Revised Date: 2012-10-31 |
Medline Journal Info:
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Nlm Unique ID: 9703165 Medline TA: J Microbiol Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 526-34 Citation Subset: IM |
Affiliation:
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Material and Chemical Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu Rd., Sec. 2, Hsinchu, 30011, Taiwan. ljchien@mail.mcut.edu.tw |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biofuels Biosynthetic Pathways / genetics* Chlorella / genetics, metabolism* Cloning, Molecular Lipid Metabolism* Metabolic Engineering* Recombinant Proteins / genetics, metabolism Saccharomyces cerevisiae / enzymology*, genetics Triglycerides / biosynthesis* Yarrowia / enzymology*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Biofuels; 0/Recombinant Proteins; 0/Triglycerides |
| Comments/Corrections | |
Erratum In:
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J Microbiol. 2012 Aug;50(4):716 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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