| Accumulation of cell cycle regulatory proteins, p21 and p27, induced after hyperthermia in human glioma cells. | |
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MedLine Citation:
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PMID: 11719966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It was investigated whether there was a relationship between p53 p21 and p27 induction pathways in the cellular response of glioma cells to hyperthermia. Two glioma cell lines were employed. A-172 cells had the wild-type of p53, and U251 cells had the mutant-type of p53. An adenovirus harbouring wild-type p53 was also used for the overexpression. The protein induction by hyperthermia was monitored by Western blot analysis. In U251 cells, the expression of wild-type p53 and hyperthermia had an additional cytotoxic effect, but did not affect A-172 cells. Significant p21 accumulation by hyperthermia was recognized in A-172 cells, and was also recognized in p53-transduced U251 cells. On the other hand, the accumulation of p27 by hyperthermia was not seen in A-172 or U251 cells, and the exogenous expression of p53 did not affect the accumulation of p27 by hyperthermia in U251 cells. These findings suggest that the p53-p21 pathway is involved in the signal transduction after hyperthermia, rather than the p27 pathway. |
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Authors:
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T Abe; T Tamiya; Y Ono; A H Salker; K Akiyama; T Ohmoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group Volume: 17 ISSN: 0265-6736 ISO Abbreviation: Int J Hyperthermia Publication Date: 2001 Nov-Dec |
Date Detail:
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Created Date: 2001-11-26 Completed Date: 2002-05-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8508395 Medline TA: Int J Hyperthermia Country: England |
Other Details:
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Languages: eng Pagination: 499-507 Citation Subset: IM |
Affiliation:
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Department of Neurological Surgery, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Cell Cycle Proteins / metabolism* Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclins / metabolism* Genes, p53 Genetic Vectors Glioma / genetics, metabolism*, therapy* Humans Hyperthermia, Induced* Lac Operon Signal Transduction Tumor Cells, Cultured Tumor Suppressor Protein p53 / metabolism Tumor Suppressor Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Tumor Suppressor Protein p53; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27 |
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