Document Detail


Accumulation of cell cycle regulatory proteins, p21 and p27, induced after hyperthermia in human glioma cells.
MedLine Citation:
PMID:  11719966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It was investigated whether there was a relationship between p53 p21 and p27 induction pathways in the cellular response of glioma cells to hyperthermia. Two glioma cell lines were employed. A-172 cells had the wild-type of p53, and U251 cells had the mutant-type of p53. An adenovirus harbouring wild-type p53 was also used for the overexpression. The protein induction by hyperthermia was monitored by Western blot analysis. In U251 cells, the expression of wild-type p53 and hyperthermia had an additional cytotoxic effect, but did not affect A-172 cells. Significant p21 accumulation by hyperthermia was recognized in A-172 cells, and was also recognized in p53-transduced U251 cells. On the other hand, the accumulation of p27 by hyperthermia was not seen in A-172 or U251 cells, and the exogenous expression of p53 did not affect the accumulation of p27 by hyperthermia in U251 cells. These findings suggest that the p53-p21 pathway is involved in the signal transduction after hyperthermia, rather than the p27 pathway.
Authors:
T Abe; T Tamiya; Y Ono; A H Salker; K Akiyama; T Ohmoto
Related Documents :
23711386 - Mdr-abc transporters: biomarkers in rheumatoid arthritis.
19861496 - Loss of the mammalian apc/c activator fzr1 shortens g1 and lengthens s phase but has li...
11991656 - The coupling of vinculin to the cytoskeleton is not essential for mechano-chemical sign...
16247456 - Defect in serine 46 phosphorylation of p53 contributes to acquisition of p53 resistance...
21896636 - The sda/gm2-glycan is a carbohydrate marker of porcine primordial germ cells and of a s...
21556596 - Studies on the effect of suramin on cell-proliferation of epithelial tissue renewal in ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group     Volume:  17     ISSN:  0265-6736     ISO Abbreviation:  Int J Hyperthermia     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2001-11-26     Completed Date:  2002-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8508395     Medline TA:  Int J Hyperthermia     Country:  England    
Other Details:
Languages:  eng     Pagination:  499-507     Citation Subset:  IM    
Affiliation:
Department of Neurological Surgery, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Cell Cycle Proteins / metabolism*
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinase Inhibitor p27
Cyclins / metabolism*
Genes, p53
Genetic Vectors
Glioma / genetics,  metabolism*,  therapy*
Humans
Hyperthermia, Induced*
Lac Operon
Signal Transduction
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / metabolism
Tumor Suppressor Proteins / metabolism*
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Tumor Suppressor Protein p53; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A hybrid equation for simulation of perfused tissue during thermal treatment.
Next Document:  Potentiation of the anti-tumour effect of hyperthermia by combining with the vascular targeting agen...