Document Detail

Accumulation and Identification of Angiotensin-converting Enzyme Inhibitory Peptides from Wheat Germ.
MedLine Citation:
PMID:  21381782     Owner:  NLM     Status:  Publisher    
The incubation conditions of wheat germ for Angiotensin I-Converting Enzyme inhibitory activity (ACEI) elevation and peptide accumulation were investigated, and five ACE inhibitory peptides were obtained. The effect of individual factor such as incubation time, temperature, initial pH and liquid to solid ratio on ACEI and peptide concentration of incubation medium was evaluated, respectively. The combinations of four factors were further optimized using a Box-Behnken design. Under the best incubation condition (pH 4.4 with a liquid to solid ratio 8.14 ml/g at temperature 47 °C, for 7 h), maximum ACEI (92.16%) and peptide concentration (88.12 mg/g) were obtained, which were 6.2-folds and 2.4-folds respectively comparing to the un-incubated wheat germ. After being purified, five ACE inhibitory peptides, VEV, W, NPPSV, QV, and AMY, were identified by LC/MS/MS. The IC50 of which were 115.20, 94.87, 40.56, 26.82 and 5.86 μM, respectively.
Runqiang Yang; Yu Zou; Nanjing Yu; Zhenxin Gu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-8
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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