Document Detail

Accumulation of DNA damage in pre- and posthepatectomized liver of aged rats.
MedLine Citation:
PMID:  10580689     Owner:  NLM     Status:  MEDLINE    
Although the majority of the literature supports the concept that an accumulation of DNA damage or a modification in the DNA structure of postmitotic cells occurs with increasing age, there are also several reports that show no DNA changes in these cells with increasing age. In the study reported here, two components of the DNA damage hypothesis of aging were tested. Young (4-6 months) and old (18-20 month) unirradiated or irradiated, pre- and posthepatectomized male Fisher 344 rats were killed, and the posterior lateral lobe of the liver removed. Single cell/nuclei suspensions were made, and the DNA damage accumulated with age or remaining at various times after irradiation was measured using the alkaline elution technique. The results demonstrate that, 1) DNA damage accumulates in rat liver cells with age, and 2) liver cells repair their radiation-induced DNA damage slower in posthepatectomized, but not prehepatectomized aged rats.
F Kalandarishvili; K T Wheeler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  110     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  2000-02-01     Completed Date:  2000-02-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  25-35     Citation Subset:  IM    
Department of Radiology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
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MeSH Terms
Aging / genetics*,  metabolism,  radiation effects
DNA / radiation effects
DNA Damage*
DNA Repair / genetics,  radiation effects
Liver / metabolism*,  radiation effects,  surgery
Rats, Inbred F344
Grant Support
Reg. No./Substance:

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