Document Detail

Accessory role of autologous T lymphocytes and adherent cells for the in vitro proliferation of T-cell colony-forming cells from patients with T-cell acute lymphoblastic leukemia.
MedLine Citation:
PMID:  3873585     Owner:  NLM     Status:  MEDLINE    
Peripheral blood T colony-forming cells (T-CFC) from patients with T-cell malignancies can proliferate in methylcellulose in the absence of added growth factors or mitogenic stimulation. Mononuclear cells (MNC) from 7 patients with T-cell acute lymphoblastic leukemia were separated into cells forming rosettes with sheep erythrocytes (E+) or not (E-). E- cells were further depleted by complement-mediated cytotoxicity with OKT3 monoclonal antibody (E-OKT3- cells). The study of their spontaneous T-cell colony-forming ability suggested that proliferation of T-CFC in the absence of added growth factors requires cellular cooperation because: (1) No colony growth was observed at low cell concentrations (up to 2 X 10(4) cells/ml) whereas at higher cell densities the number of colonies increased exponentially; (2) The plating efficiency from unfractionated MNC was higher than that from E-OKT3- or E+ cells. Irradiated autologous E+ cells enhanced the plating efficiency from blast-enriched cell fractions (E-OKT3-) when co-cultured either directly in methylcellulose or separately in a two layer assay (agar-methylcellulose), suggesting that their activity could be due to diffusible factors; (3) Adherent-cell depletion of MNC decreased colony formation. Autologous irradiated adherent cells were able to restore the plating efficiency from MNCA- cells when co-cultured directly in methylcellulose but not in separate layers; however, media conditioned by patients' A+ cells could enhance the colony growth from patients' MNCA- cells, indicating that their activity could also be mediated by constitutively released soluble factors.
V Georgoulias; C Jasmin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  9     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1985  
Date Detail:
Created Date:  1985-06-28     Completed Date:  1985-06-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  357-67     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Cell Adhesion
Colony-Forming Units Assay*
Colony-Stimulating Factors / pharmacology
Growth Substances / metabolism
Leukemia, Lymphoid / metabolism,  pathology*
Lymphocyte Activation
Organic Chemicals
T-Lymphocytes / metabolism,  pathology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Colony-Stimulating Factors; 0/Growth Substances; 0/Organic Chemicals; 0/colony promoting activity

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