Document Detail


An accessory protein required for anchoring and assembly of amyloid fibres in B. subtilis biofilms.
MedLine Citation:
PMID:  21477127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells within Bacillus subtilis biofilms are held in place by an extracellular matrix that contains cell-anchored amyloid fibres, composed of the amyloidogenic protein TasA. As biofilms age they disassemble because the cells release the amyloid fibres. This release appears to be the consequence of incorporation of D-tyrosine, D-leucine, D-tryptophan and D-methionine into the cell wall. Here, we characterize the in vivo roles of an accessory protein TapA (TasA anchoring/assembly protein; previously YqxM) that serves both to anchor the fibres to the cell wall and to assemble TasA into fibres. TapA is found in discrete foci in the cell envelope and these foci disappear when cells are treated with a mixture of D-amino acids. Purified cell wall sacculi retain a functional form of this anchoring protein such that purified fibres can be anchored to the sacculi in vitro. In addition, we show that TapA is essential for the proper assembly of the fibres. Its absence results in a dramatic reduction in TasA levels and what little TasA is left produces only thin fibres that are not anchored to the cell.
Authors:
Diego Romero; Hera Vlamakis; Richard Losick; Roberto Kolter
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-05
Journal Detail:
Title:  Molecular microbiology     Volume:  80     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-27     Completed Date:  2011-09-21     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1155-68     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Amyloid / chemistry,  genetics,  metabolism*
Bacillus subtilis / chemistry,  genetics,  physiology*
Bacterial Proteins / chemistry,  genetics,  metabolism*
Biofilms
Cell Wall / chemistry,  genetics,  metabolism
Gene Expression Regulation, Bacterial
Operon
Protein Transport
Grant Support
ID/Acronym/Agency:
GM18546/GM/NIGMS NIH HHS; GM58213/GM/NIGMS NIH HHS; R01 GM018568/GM/NIGMS NIH HHS; R01 GM018568-39/GM/NIGMS NIH HHS; R01 GM058213/GM/NIGMS NIH HHS; R01 GM058213-14/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amyloid; 0/Bacterial Proteins
Comments/Corrections
Comment In:
Mol Microbiol. 2011 Jun;80(5):1133-6   [PMID:  21488983 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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