| Accessing the hidden majority of marine natural products through metagenomics. | |
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MedLine Citation:
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PMID: 21542088 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tiny marine animals represent an untapped reservoir for undiscovered, bioactive natural products. However, their small size and extreme chemical variability preclude traditional chemical approaches to discovering new bioactive compounds. Here, we use a metagenomic method to directly discover and rapidly access cyanobactin class natural products from these variable samples, and provide proof-of-concept for genome-based discovery and supply of marine natural products. We also address practical optimization of complex, multistep ribosomal peptide pathways in heterologous hosts, which is still very challenging. The resulting methods and concepts will be applicable to ribosomal peptide and other biosynthetic pathways. |
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Authors:
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Mohamed S Donia; Duane E Ruffner; Sheng Cao; Eric W Schmidt |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-05-03 |
Journal Detail:
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Title: Chembiochem : a European journal of chemical biology Volume: 12 ISSN: 1439-7633 ISO Abbreviation: Chembiochem Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-16 Completed Date: 2011-09-02 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: Germany |
Other Details:
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Languages: eng Pagination: 1230-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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College of Pharmacy, University of Utah, Salt Lake City, UT 84112, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Biological Agents / chemistry*, isolation & purification Drug Discovery* Metagenomics* Molecular Sequence Data Molecular Structure Oceans and Seas |
| Grant Support | |
ID/Acronym/Agency:
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GM071425/GM/NIGMS NIH HHS; GM071425-S1/GM/NIGMS NIH HHS; R01 GM071425/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Agents |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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