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Acceptor activity of hypermethylated E. coli tRNAf-Met.
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MedLine Citation:
PMID:  4606167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The acceptor activity of normal E. coli tRNA(Met) (f) was compared with that of a preparation with surplus methyl groups introduced by a crude methylase preparation from rat hepatoma. No changes in charging were detected when the aminoacylation was carried out in a homologous system. The data indicate that neither the surplus methyl groups by themselves, nor the eventual changes in spatial arrangement are essential for charging of E. coli tRNA(Met) (f).
Authors:
L P Shershneva; T V Venkstern; A A Bayev
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  1     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1974 Feb 
Date Detail:
Created Date:  1974-12-19     Completed Date:  1974-12-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  235-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acyl-tRNA Synthetases / metabolism
Base Sequence
Carbon Radioisotopes
Chromatography, Thin Layer
Escherichia coli / enzymology,  metabolism*
Formic Acids
Isotope Labeling
Methionine / metabolism
Methylation
Nucleic Acid Conformation
Oligonucleotides / analysis
RNA, Bacterial / metabolism*
RNA, Transfer / metabolism*
Ribonucleotides / analysis
S-Adenosylmethionine / metabolism
Spectrophotometry, Ultraviolet
Time Factors
Tritium
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Formic Acids; 0/Oligonucleotides; 0/RNA, Bacterial; 0/Ribonucleotides; 10028-17-8/Tritium; 29908-03-0/S-Adenosylmethionine; 63-68-3/Methionine; 9014-25-9/RNA, Transfer; EC 6.1.1.-/Amino Acyl-tRNA Synthetases
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Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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? 1974 Information Retrieval Ltd 1 Falconberg Court London W1V 5FG England
Print publication date: Month: 02 Year: 1974
Volume: 1 Issue: 2
First Page: 235 Last Page: 243
ID: 343342
PubMed Id: 4606167
PubMed Id: 4606167

Acceptor activity of hypermethylated E. coli tRNAMetf
L.P. Shershneva
T.V. Venkstern
A.A. Bayev
Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow, USSR



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