Document Detail


Acceleration of functional maturation and differentiation of neonatal porcine islet cell monolayers shortly in vitro cocultured with microencapsulated sertoli cells.
MedLine Citation:
PMID:  21048849     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The limited availability of cadaveric human donor pancreata as well as the incomplete success of the Edmonton protocol for human islet allografts fasten search for new sources of insulin the producing cells for substitution cell therapy of insulin-dependent diabetes mellitus (T1DM). Starting from isolated neonatal porcine pancreatic islets (NPIs), we have obtained cell monolayers that were exposed to microencapsulated monolayered Sertoli cells (ESCs) for different time periods (7, 14, 21 days). To assess the development of the cocultured cell monolayers, we have studied either endocrine cell phenotype differentiation markers or c-kit, a hematopoietic stem cell marker, has recently been involved with growth and differentiation of β-cell subpopulations in human as well as rodent animal models. ESC which were found to either accelerate maturation and differentiation of the NPIs β-cell phenotype or identify an islet cell subpopulation that was marked positively for c-kit. The insulin/c-kit positive cells might represent a new, still unknown functionally immature β-cell like element in the porcine pancreas. Acceleration of maturation and differentiation of our NPI cell monolayers might generate a potential new opportunity to develop insulin-producing cells that may suite experimental trials for cell therapy of T1DM.
Authors:
Francesca Mancuso; Mario Calvitti; Giovanni Luca; Claudio Nastruzzi; Tiziano Baroni; Stefania Mazzitelli; Ennio Becchetti; Iva Arato; Carlo Boselli; Monique D Ngo Nselel; Riccardo Calafiore
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Publication Detail:
Type:  Journal Article     Date:  2009-11-19
Journal Detail:
Title:  Stem cells international     Volume:  2010     ISSN:  1687-9678     ISO Abbreviation:  Stem Cells Int     Publication Date:  2010  
Date Detail:
Created Date:  2010-11-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101535822     Medline TA:  Stem Cells Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  587213     Citation Subset:  -    
Affiliation:
Section of Internal Medicine and Endocrine and Metabolic Sciences, Department of Internal Medicine, University of Perugia, 06126 Perugia, Italy.
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