| Acceleration of diabetic-wound healing with PEGylated rhaFGF in healing-impaired streptozocin diabetic rats. | |
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MedLine Citation:
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PMID: 22092801 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Molecular modification with polyethylene glycol (PEGylation) is an effective approach to improve protein biostability, in vivo lifetime and therapeutic potency. In the present study, the recombinant human acid fibroblast growth factor (rhaFGF) was site-selectively PEGylated with 20 kDa mPEG-butyraldehyde. Mono-PEGylated rhaFGF was purified to near homogeneity by Sephadex G 25-gel filtration followed by a Heparin Sepharose TM CL-6B affinity chromatography. PEGylated rhaFGF has less effect than the native rhaFGF on the stimulation of 3T3 cell proliferation in vitro; however, its relative thermal stability at normal physiological temperature and structural stability were significantly enhanced, and its half-life time in vivo was significantly extended. Then, the physiological function of PEGylated rhaFGF on diabetic-wound healing was evaluated in type 1 diabetic Sprague Dawley rats. The results showed that, compared with the group of animal treated with native rhaFGF, the group treated with PEGylated rhaFGF exhibited better therapeutic efficacy with shorter healing time, quicker tissue collagen generation, earlier and higher transforming growth factor (TGF)-β expression, and dermal cell proliferation. In addition, in vivo analysis showed that both native and PEGylated rhaFGF were more effective in the wound healing in the diabetic group compared with the nondiabetic one. Taken together, these results suggest that PEGylation of rhaFGF could be a more effective approach to the pharmacological and therapeutic application of native rhaFGF. |
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Authors:
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Zhifeng Huang; Meifei Lu; Guanghui Zhu; Hongchang Gao; Liyun Xie; Xiaoqin Zhang; Chaohui Ye; Yan Wang; Chuanchuan Sun; Xiaokun Li |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society Volume: 19 ISSN: 1524-475X ISO Abbreviation: Wound Repair Regen Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9310939 Medline TA: Wound Repair Regen Country: United States |
Other Details:
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Languages: eng Pagination: 633-44 Citation Subset: IM |
Copyright Information:
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© 2011 by the Wound Healing Society. |
Affiliation:
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Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, China; College of Chemistry, Nanjing University of Science and Technology, Nanjing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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