Document Detail

Acceleration of adventitious shoots by interaction between exogenous hormone and adenine sulphate in Althaea officinalis L.
MedLine Citation:
PMID:  23001529     Owner:  NLM     Status:  MEDLINE    
In the current study attempts were made to investigate the effects of three different phases of callus induction followed by adventitious regeneration from leaf segments (central and lateral vein). Callus induction was observed in Murashige and Skoog's (MS) medium supplemented with 15.0 μM 2,4-dichloro phenoxy acetic acid (2,4-D). Adventitious shoot buds formation was achieved on MS medium supplemented with 7.5 μM 2,4-D and 20.0 μM AdS in liquid medium as it induced 19.2 ± 0.58 buds in central vein explants. Addition of different growth regulators (cytokinins-6-benzyladenine, kinetin and 2-isopentenyl adenine alone or in combination with auxins-indole-3-acetic acid, indole-3-butyric acid and α-naphthalene acetic acid, improved the shoot regeneration efficiency, in which 5.0 μM 6-benzyl adenine along with 0.25 μM α-naphthalene acetic acid was shown to be the most effective medium for maximum shoot regeneration (81.3 %) with 24.6 number of shoots and 4.4 ± 0.08 cm shoot length per explant. Leaf culture of central veins led to better shoot formation capacity in comparison to lateral vein. Rooting was readily achieved on the differentiated shoots on 1/2 MS medium augmented with 20.0 μM indole-3-butyric acid. The plants were successfully hardened off in sterile soilrite followed by their establishment in garden soil with 80 % survival rate.
Ruphi Naz; M Anis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-23
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  168     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-05-30     Revised Date:  2013-09-13    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1239-55     Citation Subset:  IM    
Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India.
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MeSH Terms
Adenine / analogs & derivatives*,  pharmacology
Adventitia / growth & development
Althaea* / drug effects,  growth & development
Cell Culture Techniques
Plant Growth Regulators / pharmacology*
Plant Leaves / cytology,  drug effects
Plant Shoots / drug effects,  growth & development*
Regeneration / drug effects
Reg. No./Substance:
0/Plant Growth Regulators; 73-24-5/Adenine

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