Document Detail


Accelerated thrombolysis in a rabbit model of carotid artery thrombosis with liposome-encapsulated and microencapsulated streptokinase.
MedLine Citation:
PMID:  12876627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study compares the efficacy of two formulations of encapsulated streptokinase to streptokinase in a rabbit model of carotid artery thrombosis. Arterial thrombosis followed the injection of thrombin mixed with autologous whole blood into a carotid artery of New Zealand white rabbits. Thirty minutes after the confirmation of an occlusive thrombus, one of four streptokinase formulations was infused at a dosage of 6000 IU/kg into the jugular vein. Free streptokinase (FREE SK) was compared to identical dosages of streptokinase encapsulated in a liposome (LESK), streptokinase entrapped in a water-soluble polymer (MESK), and free streptokinase admixed with blank microparticles (FREE SK + BLANK). Carotid arterial blood flow was determined by pulsed Doppler flowmetry to confirm clot formation and reperfusion. Two hours after drug infusion, the rabbits were killed and the residual thrombus mass was determined. Compared to FREE SK (74.5 +/- 16.9 min; mean +/- SEM), LESK demonstrated significantly reduced reperfusion times (19.3 +/- 4.6 min) while MESK exhibited even greater improvement (7.3 +/- 1.6 min). FREE SK + BLANK showed no statistical improvement versus FREE SK. LESK and MESK also resulted in reduced residual clot mass and greater return of arterial blood flow. These studies suggest that encapsulation of streptokinase offers a potential method of improved fibrinolytic treatment for patients with clot-based disorders. MESK performed slightly better than LESK with improved production and storage characteristics.
Authors:
J Kent Leach; Edgar A O'Rear; Eugene Patterson; Yiwei Miao; Arthur E Johnson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  90     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-23     Completed Date:  2004-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  64-70     Citation Subset:  IM    
Affiliation:
School of Engineering and Materials Science, University of Oklahoma, Norman, Oklahoma, 73019, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Artery Thrombosis / drug therapy*
Drug Carriers
Drug Compounding
Drug Evaluation, Preclinical
Female
Fibrinolytic Agents / administration & dosage*,  therapeutic use
Liposomes
Male
Microspheres
Models, Animal
Polyethylene Glycols
Rabbits
Recurrence
Streptokinase / administration & dosage*,  therapeutic use
Thrombin / toxicity
Thrombolytic Therapy*
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Fibrinolytic Agents; 0/Liposomes; 0/Polyethylene Glycols; EC 3.4.-/Streptokinase; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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