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Accelerated nerve regeneration using induced pluripotent stem cells in chitin-chitosan-gelatin scaffolds with inverted colloidal crystal geometry.
MedLine Citation:
PMID:  23261585     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Polymeric biomaterials with regular pore structure can generate distinctive properties in various biomedical applications. This study presents scaffolds comprising chitin, chitosan, and gelatin with pore geometry of inverted colloidal crystals (ICC) for guiding differentiation of induced pluripotent stem (iPS) cells toward neurons. Chitin-chitosan-gelatin ICC scaffolds were fabricated by self-assembly, crosslinking, infiltration, dehydration, and particle leaching. The results revealed that ethanol as a dispersion medium yielded a higher regularity of colloidal template than ethylene glycol. For the adhesion of iPS cells, freeform constructs were more efficient than ICC constructs. The reverse was true for the viability of iPS cells. The quantity of stage-specific embryonic surface antigen-1 in cultured freeform construct was larger than that in cultured ICC construct, indicating that the former preserved more phenotypic characteristics of iPS cells than the latter. Moreover, β III tubulin-identified region in ICC construct was larger than that in freeform construct, demonstrating that the differentiation of iPS cells toward neurons in ICC construct was faster than that in freeform construct. ICC topography in chitin-chitosan-gelatin scaffolds can accelerate neuronal differentiation of iPS cells.
Authors:
Yung-Chih Kuo; Ching-Chun Lin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  103C     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-12-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  -    
Other Details:
Languages:  ENG     Pagination:  595-600     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, ROC. Electronic address: chmyck@ccu.edu.tw.
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