| Accelerated lipid absorption in mice overexpressing intestinal SR-BI. | |
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MedLine Citation:
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PMID: 16421100 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dietary cholesterol absorption contributes to a large part of the circulating cholesterol. However, the mechanism of sterol intestinal uptake is not clearly elucidated. Scavenger receptor class B type I (SR-BI), major component in the control of cholesterol homeostasis, is expressed in the intestine, but its role in this organ remains unclear. We have generated transgenic mice overexpressing SR-BI primarily in the intestine by using the mouse SR-BI gene under the control of intestinal specific "apoC-III enhancer coupled with apoA-IV promoter." We found SR-BI overexpression with respect to the natural protein along the intestine and at the top of the villosities. After a meal containing [(14)C]cholesterol and [(3)H]triolein, SR-BI transgenic mice presented a rise in intestinal absorption of both lipids that was not due to a defect in chylomicron clearance nor to a change in the bile flow or the bile acid content. Nevertheless, SR-BI transgenic mice showed a decrease of total cholesterol but an increase of triglyceride content in plasma without any change in the high density lipoprotein apoA-I level. Thus, we described for the first time a functional role in vivo for SR-BI in cholesterol but also in triglyceride intestinal absorption. |
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Authors:
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Florence Bietrix; Daoguang Yan; Michel Nauze; Corinne Rolland; Justine Bertrand-Michel; Christine Coméra; Stephane Schaak; Ronald Barbaras; Albert K Groen; Bertrand Perret; François Tercé; Xavier Collet |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-18 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 281 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-13 Completed Date: 2006-05-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7214-9 Citation Subset: IM |
Affiliation:
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INSERM U 563, Centre de Physiopathologie de Toulouse Purpan, Département Lipoprotéines et Médiateurs Lipidiques, IFR30 and Université Paul Sabatier, 31024 Toulouse Cedex 3, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Apolipoproteins / chemistry Bile Acids and Salts / metabolism Cell Membrane / metabolism Cholesterol / chemistry, metabolism Chylomicrons / chemistry DNA, Complementary / metabolism Homeostasis Immunohistochemistry Intestines / chemistry, metabolism* Lipids / chemistry* Lipoproteins / chemistry Liver / metabolism Mice Mice, Transgenic Promoter Regions, Genetic Receptors, Lipoprotein / metabolism Receptors, Scavenger / chemistry Scavenger Receptors, Class B / metabolism* Tissue Distribution Triglycerides / metabolism Triolein / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins; 0/Bile Acids and Salts; 0/Chylomicrons; 0/DNA, Complementary; 0/Lipids; 0/Lipoproteins; 0/Receptors, Lipoprotein; 0/Receptors, Scavenger; 0/Scarb1 protein, mouse; 0/Scavenger Receptors, Class B; 0/Triglycerides; 122-32-7/Triolein; 57-88-5/Cholesterol |
| Comments/Corrections | |
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