Document Detail

Accelerated fat cell aging links oxidative stress and insulin resistance in adipocytes.
MedLine Citation:
PMID:  23385819     Owner:  NLM     Status:  In-Data-Review    
Telomere shortening is emerging as a biological indicator of accelerated aging and aging-related diseases including type 2 diabetes. While telomere length measurements were largely done in white blood cells, there is lack of studies on telomere length in relation to oxidative stress in target tissues affected in diabetes. Therefore, the aim of this study is to induct oxidative stress in adipocytes and to test whether these adipocytes exhibit shortened telomeres, senescence and functional impairment. 3T3-L1 adipocytes were subjected to oxidative stress and senescence induction by a variety of means for 2 weeks (exogenous application of H2O2, glucose oxidase, asymmetric dimethylarginine (ADMA) and glucose oscillations). Cells were probed for reactive oxygen species generation (ROS), DNA damage, mRNA and protein expression of senescent and pro-inflammatory markers, telomere length and glucose uptake. Compared to untreated cells, both ROS generation and DNA damage were significantly higher in cells subjected to oxidative stress and senescence. Adipocytes subjected to oxidative stress also showed shortened telomeres and increased mRNA and protein expression of p53, p21, TNF alpha and IL-6. Senescent cells were also characterized by decreased levels of adiponectin and impaired glucose uptake. Briefly, adipocytes under oxidative stress exhibited increased ROS generation, DNA damage, shortened telomeres and switched to senescent/pro-inflammatory phenotype with impaired glucose uptake.
Finny Monickaraj; Sankaramoorthy Aravind; Pichamoorthy Nandhini; Paramasivam Prabu; Chandrakumar Sathishkumar; Viswanathan Mohan; Muthuswamy Balasubramanyam
Related Documents :
24241789 - Hyperglycaemia has a greater impact on left ventricle function in south asians than eur...
16483759 - Poly(vinylpyrrolidone)-doped nitric oxide-releasing xerogels as glucose biosensor membr...
24427529 - Multiscale modelling of insulin secretion during an intravenous glucose tolerance test.
24520839 - Absence of clinical relationship between oxidized low density lipoproteins and diabetic...
24831859 - Diabetes mellitus prevalence and control in sleep disordered breathing: the european sl...
24309189 - Longitudinal changes in estimated and measured gfr in type 1 diabetes.
3314469 - Glycemic response in children with insulin-dependent diabetes mellitus after high- or l...
16361659 - Scanning laser entoptic perimetry for the detection of visual defects associated with d...
3669189 - A study of bladder dysfunction during streptozotocin-induced diabetes in the rat using ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biosciences     Volume:  38     ISSN:  0973-7138     ISO Abbreviation:  J. Biosci.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  113-22     Citation Subset:  IM    
Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control, IDF Centre of Education, Gopalapuram, Chennai 600 086, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2.
Next Document:  Derivation, characterization and retinal differentiation of induced pluripotent stem cells.