Document Detail


Accelerated evolution and coevolution drove the evolutionary history of AGPase sub-units during angiosperm radiation.
MedLine Citation:
PMID:  22307567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: ADP-glucose pyrophosphorylase (AGPase) is a key enzyme of starch biosynthesis. In the green plant lineage, it is composed of two large (LSU) and two small (SSU) sub-units encoded by paralogous genes, as a consequence of several rounds of duplication. First, our aim was to detect specific patterns of molecular evolution following duplication events and the divergence between monocotyledons and dicotyledons. Secondly, we investigated coevolution between amino acids both within and between sub-units.
METHODS: A phylogeny of each AGPase sub-unit was built using all gymnosperm and angiosperm sequences available in databases. Accelerated evolution along specific branches was tested using the ratio of the non-synonymous to the synonymous substitution rate. Coevolution between amino acids was investigated taking into account compensatory changes between co-substitutions.
KEY RESULTS: We showed that SSU paralogues evolved under high functional constraints during angiosperm radiation, with a significant level of coevolution between amino acids that participate in SSU major functions. In contrast, in the LSU paralogues, we identified residues under positive selection (1) following the first LSU duplication that gave rise to two paralogues mainly expressed in angiosperm source and sink tissues, respectively; and (2) following the emergence of grass-specific paralogues expressed in the endosperm. Finally, we found coevolution between residues that belong to the interaction domains of both sub-units.
CONCLUSIONS: Our results support the view that coevolution among amino acid residues, especially those lying in the interaction domain of each sub-unit, played an important role in AGPase evolution. First, within SSU, coevolution allowed compensating mutations in a highly constrained context. Secondly, the LSU paralogues probably acquired tissue-specific expression and regulatory properties via the coevolution between sub-unit interacting domains. Finally, the pattern we observed during LSU evolution is consistent with repeated sub-functionalization under 'Escape from Adaptive Conflict', a model rarely illustrated in the literature.
Authors:
Jonathan Corbi; Julien Y Dutheil; Catherine Damerval; Maud I Tenaillon; Domenica Manicacci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-02
Journal Detail:
Title:  Annals of botany     Volume:  109     ISSN:  1095-8290     ISO Abbreviation:  Ann. Bot.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-27     Completed Date:  2012-09-06     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  693-708     Citation Subset:  IM    
Affiliation:
CNRS, UMR Génétique Végétale, Gif sur Yvette, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Angiosperms / enzymology,  genetics*,  radiation effects*
Base Sequence
Evolution, Molecular*
Gene Duplication
Gene Expression Regulation, Plant
Genes, Plant
Genetic Variation
Glucose-1-Phosphate Adenylyltransferase / metabolism*
Molecular Sequence Data
Phylogeny
Selection, Genetic
Starch / biosynthesis
Chemical
Reg. No./Substance:
9005-25-8/Starch; EC 2.7.7.27/Glucose-1-Phosphate Adenylyltransferase
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