| Acanthamoeba castellanii cysts: new ultrastructural findings. | |
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MedLine Citation:
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PMID: 23319266 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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During Acanthamoeba castellanii trophozoite-cysts differentiation, four morphological stages were identified by scanning electron microscopy: trophozoite, precyst, immature cysts, and mature cysts. Fluorescence microscopy reveals the presence of small cumulus of actin in the cytoplasm of precysts after treatment with rhodamine phalloidin. By the contrary, in mature cysts, fluorescence was not observed. However, when excystation was induced, large fluorescent patches were present. By transmission electron microscopy, encysting amebas showed small cytoplasmic vesicles containing fibrillar material, surrounded by a narrow area of thin fibrils. Similar appearance was observed in pseudopods and phagocytic invaginations. In addition, large aggregates of rod-shape elements, similar to the chromatoid bodies, described in other amebas, were present in the cytoplasm. These cysts presented large areas with orange fluorescence after treatment with acridine orange. |
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Authors:
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Bibiana Chávez-Munguía; Lizbeth Salazar-Villatoro; Anel Lagunes-Guillén; Maritza Omaña-Molina; Martha Espinosa-Cantellano; Adolfo Martínez-Palomo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-15 |
Journal Detail:
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Title: Parasitology research Volume: - ISSN: 1432-1955 ISO Abbreviation: Parasitol. Res. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Av. IPN 2508, Zacatenco, 07360, Mexico City, Mexico, bchavez@cinvestav.mx. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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