Document Detail


Acanthamoeba castellanii: cellular changes induced by chlorination.
MedLine Citation:
PMID:  20034490     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chlorination is a well-known disinfection method, used in water treatment to inactivate various microorganisms, it induces numerous cellular changes. Even though Acanthamoebae are frequently found in water, the cellular changes induced in Acanthamoebae have not been described in the literature. Acanthamoebae are pathogenic amoebae and may provide a reservoir for pathogenic bacteria such as Legionellapneumophila; it is consequently important to understand the response of this amoeba to chlorination, and our study was indeed aimed at examining cellular changes in Acanthamoebae following chlorination. Acanthamoeba trophozoites were treated at various chlorine concentrations (1-5mg/L). A 3-log reduction in Acanthamoebae population was achieved with 5mg/L of free chlorine. Confocal microscopy and flow cytometry experiments indicated that chlorination induced cell permeabilization, size reduction and likely intracellular thiol concentration. Our data show that among the non-cultivable cells some remained impermeabilized (negative staining with propidium iodide), thereby suggesting that these cells might remained viable. A similar state is described in other microorganisms as a VBNC (viable but not cultivable) state. Electron microscopy observations illustrate drastic morphological changes: the pseudopods disappeared and subcellular components, such as mitochondrion, were pronouncedly affected. In conclusion, depending on the concentration used, chlorination leads to many cellular effects on Acanthamoeba that could well arise in cell inactivation.
Authors:
Emerancienne Mogoa; Charles Bodet; Bernard Legube; Yann Héchard
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-23
Journal Detail:
Title:  Experimental parasitology     Volume:  126     ISSN:  1090-2449     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-102     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
Laboratoire de Chimie et Microbiologie de l'Eau, UMR CNRS 6008, Université de Poitiers, 40 Avenue du recteur Pineau, 86022 Poitiers Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Acanthamoeba castellanii / cytology,  drug effects*,  ultrastructure
Cell Membrane Permeability / drug effects
Chlorine / administration & dosage,  chemistry,  pharmacology*
Disinfection / methods*
Flow Cytometry
Halogenation
Microscopy, Confocal
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Sulfhydryl Compounds / metabolism
Water Purification / methods*
Chemical
Reg. No./Substance:
0/Sulfhydryl Compounds; 7782-50-5/Chlorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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