Document Detail


Abundance and diversity of heterotrophic bacterial cells assimilating phosphate in the subtropical North Atlantic Ocean.
MedLine Citation:
PMID:  20545744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microorganisms play key roles in the cycles of carbon and nutrients in the ocean, and identifying the extent to which specific taxa contribute to these cycles will establish their ecological function. We examined the use of (33)P-phosphate to identify heterotrophic bacteria actively involved in the cycling of phosphate, an essential inorganic nutrient. Seawater from the sub-tropical North Atlantic Ocean was incubated with (33)P-phosphate and analysed by microautoradiography to determine the proportion and diversity of the bacterial community-assimilating phosphate. Complementary incubations using (3)H-leucine and (3)H-thymidine were also conducted. We found that a higher proportion of total heterotrophic bacterial cells in surface water samples assimilated phosphate compared with leucine or thymidine. Bacteria from all of the phylogenetic groups we identified by CARD-FISH were able to assimilate phosphate, although the abundances of cells within each group did not scale directly with the number found to assimilate phosphate. Furthermore, a significantly higher proportion of Alphaproteobacteria, Gammaproteobacteria and Cytophaga-like cells assimilated phosphate compared with leucine or thymidine. Our results suggest that a greater proportion of bacterial cells in surface waters are actively participating in the biogeochemical cycling of phosphorus, and possibly other elements, than is currently estimated through the use of (3)H-leucine or (3)H-thymidine.
Authors:
Krista Longnecker; Michael W Lomas; Benjamin A S Van Mooy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental microbiology     Volume:  12     ISSN:  1462-2920     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-29     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2773-82     Citation Subset:  IM    
Copyright Information:
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
Affiliation:
Woods Hole Oceanographic Institution, Marine Chemistry and Geochemistry, Woods Hole, MA 02543, USA.
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MeSH Terms
Descriptor/Qualifier:
Aquatic Organisms / classification,  metabolism*
Atlantic Ocean
Bacteria / classification,  metabolism*
Ecological and Environmental Processes
Heterotrophic Processes
Leucine / analysis,  metabolism
Phosphates / analysis,  metabolism*
Phosphorus Radioisotopes / analysis
Seawater / chemistry,  microbiology*
Thymidine / analysis,  metabolism
Water Pollutants, Chemical / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Phosphates; 0/Phosphorus Radioisotopes; 0/Water Pollutants, Chemical; 50-89-5/Thymidine; 61-90-5/Leucine

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