Document Detail

Absorption of urea through the oral mucosa and estimation of the percentage of secreted whole saliva inadvertently swallowed during saliva collection.
MedLine Citation:
PMID:  16023991     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether some of the urea added to certain chewing gums may be absorbed through the oral mucosa and whether some saliva is inadvertently swallowed during the collection of saliva elicited by the chewing of gum. DESIGN: On two occasions, 10 experienced saliva collectors made a 5 min collection of unstimulated whole saliva and then chewed gum for 10 min and during this time collected their saliva. On one occasion, they chewed one tablet of gum containing 0.5 mg of Phenol Red, a non-absorbable substance, and one tablet of a gum containing 27.3 mg of urea. On another occasion, they chewed two tablets of the Phenol Red gum. Their saliva and the chewed gum were assayed for their Phenol Red and urea contents and the totals calculated. Since saliva normally contains urea, the recovery of urea was calculated as the difference between the amounts recovered in the two collection sessions. RESULTS: The mean recovery of Phenol Red was 96.7%, but in three participants the amount recovered was less than the 95% confidence limits for assay error. The mean recovery of urea was 85.7% and in nine of the 10 participants, the amount recovered was less than the confidence limits for assay error. In all participants, the percentage urea recovery was less than that of Phenol Red. CONCLUSION: The results showed: (1) that Phenol Red appears to be a useful, non-absorbed marker for studies of drug absorption through the oral mucosa, (2) that when the salivary urea concentration is higher than that in plasma, urea may be absorbed through the oral mucosa, (3) that even experienced saliva collectors may inadvertently swallow some of the saliva they produce. This latter finding has implications for all clinical studies of saliva.
C Dawes
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-07-15
Journal Detail:
Title:  Archives of oral biology     Volume:  51     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-13     Completed Date:  2006-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  111-6     Citation Subset:  D; IM    
Department of Oral Biology, Faculty of Dentistry, University of Manitoba, 780 Bannatyne Avenue, Winnipeg MB, Canada R3E 0W2.
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MeSH Terms
Chewing Gum
Middle Aged
Mouth Mucosa / metabolism*
Phenolsulfonphthalein / diagnostic use
Saliva / metabolism*,  secretion
Specific Gravity
Specimen Handling / methods
Urea / pharmacokinetics*
Reg. No./Substance:
0/Chewing Gum; 143-74-8/Phenolsulfonphthalein; 57-13-6/Urea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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