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THE ABSORPTION SPECTRUM OF VISUAL PURPLE.
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MedLine Citation:
PMID:  19873058     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The absorption spectra of visual purple solutions extracted by various means were measured with a sensitive photoelectric spectrophotometer and compared with the classical visual purple absorption spectrum. Hardening the retinas in alum before extraction yielded visual purple solutions of much higher light transmission in the blue and violet, probably because of the removal of light-dispersing substances. Re-extraction indicated that visual purple is more soluble in the extractive than are the other colored retinal components. However, the concentration of the extractive did not affect the color purity of the extraction but did influence the keeping power. This suggests a chemical combination between the extractive and visual purple. The pH of the extractive affected the color purity of the resulting solution. Over the pH range from 5.5 to 10.0, the visual purple color purity was greatest at the low pH. Temperature during extraction was also effective, the color purity being greater the higher the temperature, up to 40 degrees C. Drying and subsequent re-dissolving of visual purple solutions extracted with digitalin freed the solution of some protein impurities and increased its keeping power. Dialysis against distilled water seemed to precipitate visual purple from solution irreversibly. None of the treatments described improved the symmetry of the unbleached visual purple absorption spectrum sufficiently for it to resemble the classical absorption spectrum. Therefore it is very likely that the classical absorption spectrum is that of the light-sensitive group only and that the absorption spectra of our purest unbleached visual purple solutions represent the molecule as a whole.
Authors:
A M Chase; C Haig
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  21     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1938 Mar 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-06-23     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  411-30     Citation Subset:  -    
Affiliation:
Laboratory of Biophysics, Columbia University, New York.
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Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1938, by The Rockefeller Institute for Medical Research
Accepted Day: 1 Month: 10 Year: 1937
Print publication date: Day: 20 Month: 3 Year: 1938
Volume: 21 Issue: 4
First Page: 411 Last Page: 430
ID: 2141953
PubMed Id: 19873058

THE ABSORPTION SPECTRUM OF VISUAL PURPLE
Aurin M. Chase
Charles Haig
From the Laboratory of Biophysics, Columbia University, New York


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