Document Detail

Absorption, metabolism, degradation and urinary excretion of rosmarinic acid after intake of Perilla frutescens extract in humans.
MedLine Citation:
PMID:  15309457     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Rosmarinic acid (RA) is a natural polyphenolic substance contained in many Lamiaceae herbs such as Perilla frutescens. Previous studies have shown RA has antioxidative and anti-inflammatory activity. However, little is known on the absorption, metabolism, degradation and excretion of RA. AIM OF THE STUDY: The aim of this study in healthy humans was to determine the absorption, metabolism, and urinary excretion of RA after a single intake of perilla extract (PE). METHOD: Six healthy men (mean age 37.2 +/- 6.2 y and mean body mass index 22.0 +/- 1.9 kg/m(2)) were enrolled in the study that was a crossover design involving single intakes of PE containing 200 mg RA and placebo with a 10 day interval between treatments. Blood samples were collected before intake and at designated time intervals, while urine samples were collected over the periods 0-6 h, 6-24 h and 24-48 h after intake. RA and its related metabolites in plasma and urine were measured by LC-MS. RESULTS: RA, methylated RA (methyl-RA), caffeic acid (CAA), ferulic acid (FA) and a trace of m-coumaric acid (COA) were detected in the urine after intake of PE. In plasma, RA, methyl-RA and FA were detected, with maximum levels obtained 0.5, 2 and 0.5 h after intake of PE, respectively. The majority of these components in both plasma and urine were present as conjugated forms (glucuronide and/or sulfated). The proportion of RA and its related metabolites excreted in the urine was 6.3 +/- 2.2% of the total dose, with approximately 75% of these components being excreted within 6 h after intake of PE. CONCLUSIONS: RA contained in PE was absorbed, conjugated and methylated following intake, with a small proportion of RA being degraded into various components, such as conjugated forms of CAA, FA and COA. These metabolites were then rapidly excreted in the urine.
Seigo Baba; Naomi Osakabe; Midori Natsume; Akiko Yasuda; Yuko Muto; Kyoko Hiyoshi; Hirohisa Takano; Toshikazu Yoshikawa; Junji Terao
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article     Date:  2004-02-18
Journal Detail:
Title:  European journal of nutrition     Volume:  44     ISSN:  1436-6207     ISO Abbreviation:  Eur J Nutr     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-02     Completed Date:  2006-08-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100888704     Medline TA:  Eur J Nutr     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Nutritional Science Center, Health and Bioscience Laboratories, Meiji Seika Kaisha, Ltd., Sakado-shi, Saitama 350-0289, Japan.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / metabolism*
Antioxidants / metabolism*
Biological Markers / metabolism
Caffeic Acids / metabolism
Catechol O-Methyltransferase / drug effects,  metabolism
Cinnamates / metabolism*,  urine
Coumaric Acids / metabolism
Cross-Over Studies
Gas Chromatography-Mass Spectrometry
Methylation / drug effects
Middle Aged
Perilla frutescens* / metabolism
Plant Extracts
Plant Structures
Reference Values
Serine Proteinase Inhibitors / metabolism*,  urine
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antioxidants; 0/Biological Markers; 0/Caffeic Acids; 0/Cinnamates; 0/Coumaric Acids; 0/Depsides; 0/Plant Extracts; 0/Serine Proteinase Inhibitors; 1135-24-6/ferulic acid; 331-39-5/caffeic acid; 537-15-5/rosmarinic acid; EC O-Methyltransferase
Erratum In:
Eur J Nutr. 2005 Feb;44(1):9

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