| Absorption, metabolism, degradation and urinary excretion of rosmarinic acid after intake of Perilla frutescens extract in humans. | |
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MedLine Citation:
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PMID: 15309457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Rosmarinic acid (RA) is a natural polyphenolic substance contained in many Lamiaceae herbs such as Perilla frutescens. Previous studies have shown RA has antioxidative and anti-inflammatory activity. However, little is known on the absorption, metabolism, degradation and excretion of RA. AIM OF THE STUDY: The aim of this study in healthy humans was to determine the absorption, metabolism, and urinary excretion of RA after a single intake of perilla extract (PE). METHOD: Six healthy men (mean age 37.2 +/- 6.2 y and mean body mass index 22.0 +/- 1.9 kg/m(2)) were enrolled in the study that was a crossover design involving single intakes of PE containing 200 mg RA and placebo with a 10 day interval between treatments. Blood samples were collected before intake and at designated time intervals, while urine samples were collected over the periods 0-6 h, 6-24 h and 24-48 h after intake. RA and its related metabolites in plasma and urine were measured by LC-MS. RESULTS: RA, methylated RA (methyl-RA), caffeic acid (CAA), ferulic acid (FA) and a trace of m-coumaric acid (COA) were detected in the urine after intake of PE. In plasma, RA, methyl-RA and FA were detected, with maximum levels obtained 0.5, 2 and 0.5 h after intake of PE, respectively. The majority of these components in both plasma and urine were present as conjugated forms (glucuronide and/or sulfated). The proportion of RA and its related metabolites excreted in the urine was 6.3 +/- 2.2% of the total dose, with approximately 75% of these components being excreted within 6 h after intake of PE. CONCLUSIONS: RA contained in PE was absorbed, conjugated and methylated following intake, with a small proportion of RA being degraded into various components, such as conjugated forms of CAA, FA and COA. These metabolites were then rapidly excreted in the urine. |
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Authors:
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Seigo Baba; Naomi Osakabe; Midori Natsume; Akiko Yasuda; Yuko Muto; Kyoko Hiyoshi; Hirohisa Takano; Toshikazu Yoshikawa; Junji Terao |
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Publication Detail:
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Type: Clinical Trial; Controlled Clinical Trial; Journal Article Date: 2004-02-18 |
Journal Detail:
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Title: European journal of nutrition Volume: 44 ISSN: 1436-6207 ISO Abbreviation: Eur J Nutr Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-02 Completed Date: 2006-08-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100888704 Medline TA: Eur J Nutr Country: Germany |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Nutritional Science Center, Health and Bioscience Laboratories, Meiji Seika Kaisha, Ltd., Sakado-shi, Saitama 350-0289, Japan. seigo_baba@meiji.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anti-Inflammatory Agents, Non-Steroidal / metabolism* Antioxidants / metabolism* Biological Markers / metabolism Caffeic Acids / metabolism Catechol O-Methyltransferase / drug effects, metabolism Cinnamates / metabolism*, urine Coumaric Acids / metabolism Cross-Over Studies Depsides Gas Chromatography-Mass Spectrometry Humans Male Methylation / drug effects Middle Aged Perilla frutescens* / metabolism Plant Extracts Plant Structures Reference Values Serine Proteinase Inhibitors / metabolism*, urine |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antioxidants; 0/Biological Markers; 0/Caffeic Acids; 0/Cinnamates; 0/Coumaric Acids; 0/Depsides; 0/Plant Extracts; 0/Serine Proteinase Inhibitors; 1135-24-6/ferulic acid; 331-39-5/caffeic acid; 537-15-5/rosmarinic acid; EC 2.1.1.6/Catechol O-Methyltransferase |
| Comments/Corrections | |
Erratum In:
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Eur J Nutr. 2005 Feb;44(1):9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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