| Absorption-based highly sensitive and reproducible biochemical oxygen demand measurement method for seawater using salt-tolerant yeast Saccharomyces cerevisiae ARIF KD-003. | |
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MedLine Citation:
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PMID: 18558133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salt-tolerant yeast Saccharomyces cerevisiae ARIF KD-003 was applied to highly sensitive and reproducible absorbance-based biochemical oxygen demand (BOD(AB-ScII)) measurement for seawater. In the previous work, we have studied the BOD(AB-ScI) method using normal Baker's yeast S. cerevisiae, and the excellent feature of the Baker's yeast as uniformly sustainable in solution could successfully be utilized. However, the BOD(AB-ScI) responses were disappeared by the existence of chloride ion as well as seawater. In the present method, uniformity in solution was also observed with S. cerevisiae ARIF KD-003, and salt-tolerance of the yeast was observed even in saturate concentration of sodium chloride. Next, characterizations of the influences of pH and incubation temperature were investigated. After optimum conditions were obtained, two calibration curves were made between 0.33 and 22 mg O2 L(-1) BOD using standard solution of glucose glutamic acid (GGA) or mixture of GGA and artificial seawater. Then, excellent reproducibility as the averages of relative standard deviation (R.S.D.(av)) in two calibration curves (nine points each) was successfully obtained at 1.10% at pure water or 1.03% at artificial seawater standard, respectively. In addition, the 3 sigma lower detection limit was calculated to be 0.07 mg O2 L(-1) BOD, and 0.11 mg O2 L(-1) BOD was experimentally detected by increase of the sample volume at 1.5-folds. The storage stability of the S. cerevisiae ARIF KD-003 was obtained at least 4 weeks. |
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Authors:
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Hideaki Nakamura; Yotaro Mogi; Hisashi Hattori; Yutaka Kita; Daisuke Hattori; Aki Yoshimura; Isao Karube |
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Publication Detail:
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Type: Journal Article Date: 2008-05-13 |
Journal Detail:
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Title: Analytica chimica acta Volume: 620 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-18 Completed Date: 2008-07-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 127-33 Citation Subset: IM |
Affiliation:
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School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. nakamura@bs.teu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Calibration Chlorides / chemistry Hydrogen-Ion Concentration Ions / chemistry Oxygen / chemistry*, metabolism* Reproducibility of Results Saccharomyces cerevisiae / chemistry*, metabolism* Salts* / chemistry Seawater* / chemistry Sensitivity and Specificity Temperature |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Ions; 0/Salts; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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