Document Detail

Absorption, accumulation and biological effects of depleted uranium in Peyer's patches of rats.
MedLine Citation:
PMID:  16978755     Owner:  NLM     Status:  MEDLINE    
The digestive tract is the entry route for radionuclides following the ingestion of contaminated food and/or water wells. It was recently characterized that the small intestine was the main area of uranium absorption throughout the gastrointestinal tract. This study was designed to determine the role played by the Peyer's patches in the intestinal absorption of uranium, as well as the possible accumulation of this radionuclide in lymphoid follicles and the toxicological or pathological consequences on the Peyer's patch function subsequent to the passage and/or accumulation of uranium. Results of experiments performed in Ussing chambers indicate that the apparent permeability to uranium in the intestine was higher (10-fold) in the mucosa than in Peyer's patches ((6.21+/-1.21 to 0.55+/-0.35)x10(-6)cm/s, respectively), demonstrating that the small intestinal epithelium was the preferential pathway for the transmucosal passage of uranium. A quantitative analysis of uranium by ICP-MS following chronic contamination with depleted uranium during 3 or 9 months showed a preferential accumulation of uranium in Peyer's patches (1355% and 1266%, respectively, at 3 and 9 months) as compared with epithelium (890% and 747%, respectively, at 3 and 9 months). Uranium was also detected in the mesenteric lymph nodes ( approximately 5-fold after contamination with DU). The biological effects of this accumulation of depleted uranium after chronic contamination were investigated in Peyer's patches. There was no induction of the apoptosis pathway after chronic DU contamination in Peyer's patches. The results indicate no change in the cytokine expression (Il-10, TGF-beta, IFN-gamma, TNF-alpha, MCP-1) in Peyer's patches and in mesenteric lymph nodes, and no modification in the uptake of yeast cells by Peyer's patches. In conclusion, this study shows that the Peyer's patches were a site of retention for uranium following the chronic ingestion of this radionuclide, without any biological consequences of such accumulation on Peyer's patch functions.
I Dublineau; S Grison; L Grandcolas; C Baudelin; C Tessier; D Suhard; S Frelon; C Cossonnet; M Claraz; J Ritt; P Paquet; P Voisin; P Gourmelon
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-17
Journal Detail:
Title:  Toxicology     Volume:  227     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-25     Completed Date:  2006-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  227-39     Citation Subset:  IM    
IRSN, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidémiologie, Laboratoire de Radiotoxicologie expérimentale, IRSN, BP 17, F-92262 Fontenay-aux-Roses Cedex, France.
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MeSH Terms
Apoptosis / drug effects
Cell Proliferation / drug effects
Cytokines / genetics,  immunology
Gene Expression / drug effects
Ileum / drug effects,  immunology,  metabolism*,  pathology
Intestinal Absorption* / drug effects
Intestinal Mucosa / drug effects,  immunology,  metabolism*,  pathology
Peyer's Patches / drug effects,  immunology,  metabolism*,  pathology
Rats, Sprague-Dawley
Time Factors
Uranyl Nitrate / pharmacokinetics*,  toxicity
Reg. No./Substance:
0/Cytokines; 10102-06-4/Uranyl Nitrate

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