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Absolute quantitation of proteins by Acid hydrolysis combined with amino Acid detection by mass spectrometry.
MedLine Citation:
PMID:  22125141     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Amino acid analysis is among the most accurate methods for absolute quantification of proteins and peptides. Here, we combine acid hydrolysis with the addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10 fmol of protein standards with errors below 10% has been demonstrated using this method (1).
Authors:
Olga A Mirgorodskaya; Roman Körner; Yuri P Kozmin; Peter Roepstorff
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  828     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-20     Citation Subset:  IM    
Affiliation:
Research Institute of Influenza, Saint-Petersburg, Russia.
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