Document Detail


Absence of uvomorulin in a slowly compacting variant of H6 embryonal carcinoma cells.
MedLine Citation:
PMID:  2321096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When allowed to aggregate in calcium-containing medium, the H6 embryonal carcinoma cell variant named 6B(NG)C25 compacted more slowly than wild-type cells, and aggregates of hybrids between it and wild-type cells also compacted slowly, as if the variation (mutation) acted in a dominant fashion. In agreement with this, we now have found that the cell adhesion molecule uvomorulin is markedly reduced or absent in 6B(NG)C25 cells, as well as in the hybrids. A small amount of a higher-molecular-weight protein reacting with the antibody is present, which might represent residual uvomorulin migrating at a slower rate, an altered uvomorulin, the known precursor to uvomorulin, or an unrelated cross-reacting protein.
Authors:
J W Littlefield; L L Whitehouse
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell and molecular genetics     Volume:  16     ISSN:  0740-7750     ISO Abbreviation:  Somat. Cell Mol. Genet.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-30     Completed Date:  1990-04-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8403568     Medline TA:  Somat Cell Mol Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-4     Citation Subset:  IM    
Affiliation:
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Cadherins / analysis*,  metabolism
Cell Line
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Humans
Hybrid Cells / analysis,  cytology,  metabolism
Immunoblotting
Teratoma / analysis,  genetics,  pathology*
Grant Support
ID/Acronym/Agency:
CA 16754/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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