Document Detail

Absence of relation between the expression of cyclooxygenase isoforms and the synthesis of prostaglandin E2 in resident and thioglycollate-elicited macrophages in rats.
MedLine Citation:
PMID:  9674017     Owner:  NLM     Status:  MEDLINE    
Macrophages exudating into inflammatory sites (thioglycollate-elicited macrophages, TGM) have a diminished ability to synthesize prostaglandins (PG) as compared with resident peritoneal macrophages (RM). Constitutive expression of cyclooxygenase-1 (COX-1) was lower in TGM than in RM but the releasability of arachidonic acid was not significantly different. Thus, the differences in expression of COX-1 were primarily responsible for the abilities of TGM and RM to synthesize PGE2 upon calcium ionophore (CaI) stimulation. COX-1 expression in RM and TGM was also correlated with their ability to synthesize PGE2 from exogenously added arachidonic acid. When exposed to lipopolysaccharide (LPS), the induction of COX-2 and the enhancement of PGE2 synthesis upon CaI were much lower in TGM as compared with RM the releasability of arachidonic acid upon CaI stimulation was relatively unchanged in RM but was reduced in TGM Thus, in TGM as compared with RM, a lower level of COX-1 expression and a lower level of COX-2 induction, and the reduction of arachidonate releasability by LPS exposure, are mainly responsible for lower PGE2 synthetic ability upon CaI stimulation. However, the different COX-2 induction by LPS in RM and TGM was not reflected in their increase in the ability to synthesize PGE2 from exogenously added arachidonic acid.
S Watanabe; T Kobayashi; H Okuyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins & other lipid mediators     Volume:  56     ISSN:  1098-8823     ISO Abbreviation:  Prostaglandins Other Lipid Mediat.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-10-01     Completed Date:  1998-10-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9808648     Medline TA:  Prostaglandins Other Lipid Mediat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-18     Citation Subset:  IM    
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
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MeSH Terms
Arachidonic Acid / metabolism
Blotting, Western
Calcimycin / pharmacology
Chromatography, High Pressure Liquid
Dinoprostone / biosynthesis*,  blood
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Enzymologic
Immunoenzyme Techniques
Ionophores / pharmacology
Isoenzymes / biosynthesis*
Lipopolysaccharides / pharmacology
Macrophages, Peritoneal / drug effects,  metabolism*
Prostaglandin-Endoperoxide Synthases / biosynthesis*
Prostaglandins B / analysis
Rats, Sprague-Dawley
Thioglycolates / pharmacology*
Reg. No./Substance:
0/Ionophores; 0/Isoenzymes; 0/Lipopolysaccharides; 0/Prostaglandins B; 0/Thioglycolates; 13367-85-6/prostaglandin B2; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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