| Absence of the oxytocin-induced prostaglandin F2 alpha secretory response in uterus from ovariectomized ewes and activation of the response in vitro. | |
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MedLine Citation:
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PMID: 7616163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxytocin-induced prostaglandin F2 alpha (PGF2 alpha) responses were measured in explants of uterus from ovariectomized ewes on the day of tissue collection or after culture for 72 h in the presence or absence of oestradiol-17 beta (100 nmol/l). Oxytocin receptor binding activity was 210 +/- 47 fmol [3H]oxytocin bound per mg protein in fresh tissue and 89 +/- 24 and 90 +/- 17 fmol/mg in tissue cultured with control medium or with oestradiol respectively (means +/- S.E.M.). PGF2 alpha production during the hour following oxytocin administration to freshly collected tissue was 272 +/- 77 ng/g/h compared with 193 +/- 35 ng/g/h in the absence of oxytocin. These rates were 2789 +/- 1085 and 353 +/- 135 ng/g/h after culture for 72 h in control medium and 2022 +/- 496 and 342 +/- 134 ng/g/h after culture with oestradiol. Thus oestradiol had no effect on the culture-induced maturation of the PGF2 alpha response. Short-term exposure to arachidonic acid (66 mumol/l) did not increase PGF2 alpha production in fresh tissue but significantly increased basal but not oxytocin-induced PGF2 alpha production after 72 h in culture (P < 0.05). There was an absence of oxytocin-induced inositol phosphate turnover in fresh tissue but after culture concentrations of inositol mono-, bis- and trisphosphates were all significantly increased by oxytocin (P < 0.005). Antisera directed against G-protein alpha sub-units alpha i3, alpha o, alpha q, alpha 11 and the common beta subunit, and prostaglandin H-synthase-1 detected proteins that were present before and after culture.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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E L Sheldrick; H C Flick-Smith; D E Bendall; A P Flint |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of endocrinology Volume: 145 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-08-18 Completed Date: 1995-08-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 299-305 Citation Subset: IM |
Affiliation:
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Department of Cellular Physiology, Babraham Institute, Cambridge, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / pharmacology Culture Techniques Dinoprost / secretion* Electrophoresis, Polyacrylamide Gel Female GTP-Binding Proteins / metabolism Immunoblotting Inositol Phosphates / metabolism Ovariectomy Oxytocin / pharmacology* Phosphatidylinositols / metabolism Receptors, Oxytocin / metabolism Sheep / physiology* Uterus / drug effects, metabolism, secretion* |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 0/Phosphatidylinositols; 0/Receptors, Oxytocin; 50-56-6/Oxytocin; 506-32-1/Arachidonic Acid; 551-11-1/Dinoprost; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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