Document Detail


Absence of detectable chorionic gonadotropin subunit messenger ribonucleic acids in the rat placenta throughout gestation.
MedLine Citation:
PMID:  2578949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We hypothesized that if a CG similar in structure to other glycoprotein hormones was present in the rat placenta, then mRNAs encoding its subunits would be detectable. To investigate the possible presence of a CG in the rat, we attempted to detect mRNAs encoding the alpha- and CG beta-like subunits in the placentae of timed pregnant rats by sensitive blot hybridization analyses using cloned cDNAs encoding alpha- and beta-subunits of LH derived from rat pituitary mRNA. No subunit-specific mRNAs in placentae of pregnant rats at 4-21 days gestation were detected at either high or low stringencies of hybridization. However, under similar conditions, subunit-specific mRNAs were readily observed in total pituitary RNA of normal as well as ovariectomized rats. Moreover, hybridization with a cDNA for alpha-tubulin, a major component of the cytoskeleton, yielded easily detectable bands in rat placental RNA. In addition, hybridization analysis, under low stringency conditions, of restriction enzyme digests of rat genomic DNA with a rat LH beta-cDNA, which would detect LH beta subunit-like genes, suggests the presence of a single gene that, in fact, encodes the rat LH beta-subunit. We conclude that mRNAs encoding for proteins structurally homologous to rat LH subunits are absent in the rat placenta and that only a single LH beta-like gene is present in the rat. The luteotropic activity associated with the rat placenta must be related to a gonadotropin-like hormone whose structure is dissimilar from that of CG.
Authors:
F E Carr; W W Chin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  116     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-03-27     Completed Date:  1985-03-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1151-7     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chorionic Gonadotropin / classification,  genetics*
Electrophoresis
Female
Genes
Luteinizing Hormone / classification,  genetics
Methods
Placenta / metabolism*
Pregnancy
RNA / analysis,  metabolism
RNA, Messenger / metabolism*
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
AM-25532/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/RNA, Messenger; 63231-63-0/RNA; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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