Document Detail

Absence of detectable chorionic gonadotropin subunit messenger ribonucleic acids in the rat placenta throughout gestation.
MedLine Citation:
PMID:  2578949     Owner:  NLM     Status:  MEDLINE    
We hypothesized that if a CG similar in structure to other glycoprotein hormones was present in the rat placenta, then mRNAs encoding its subunits would be detectable. To investigate the possible presence of a CG in the rat, we attempted to detect mRNAs encoding the alpha- and CG beta-like subunits in the placentae of timed pregnant rats by sensitive blot hybridization analyses using cloned cDNAs encoding alpha- and beta-subunits of LH derived from rat pituitary mRNA. No subunit-specific mRNAs in placentae of pregnant rats at 4-21 days gestation were detected at either high or low stringencies of hybridization. However, under similar conditions, subunit-specific mRNAs were readily observed in total pituitary RNA of normal as well as ovariectomized rats. Moreover, hybridization with a cDNA for alpha-tubulin, a major component of the cytoskeleton, yielded easily detectable bands in rat placental RNA. In addition, hybridization analysis, under low stringency conditions, of restriction enzyme digests of rat genomic DNA with a rat LH beta-cDNA, which would detect LH beta subunit-like genes, suggests the presence of a single gene that, in fact, encodes the rat LH beta-subunit. We conclude that mRNAs encoding for proteins structurally homologous to rat LH subunits are absent in the rat placenta and that only a single LH beta-like gene is present in the rat. The luteotropic activity associated with the rat placenta must be related to a gonadotropin-like hormone whose structure is dissimilar from that of CG.
F E Carr; W W Chin
Related Documents :
7774679 - Prolonged incubation with neuropeptide y upregulates beta-adrenoceptors yet does not ca...
2888869 - Comparative analysis of beta-1 adrenoceptor agonist and antagonist potency and selectiv...
8608849 - Relay activity of 17-beta oestradiol and diethylstilbestrol in a mouse-rat system.
2907039 - A comparison of the effects of oxodipine and nifedipine on rat vas deferens.
10536669 - Inhibitory effects of vesnarinone in the progression of myocardial damage in experiment...
23851029 - Can 18f-fdg-pet show differences in myocardial metabolism between wistar kyoto rats and...
2409939 - Effects of an acute administration of nickel upon blood glucose compartmentation in pre...
6815719 - Lung, aorta, and platelet metabolism of 14c-arachidonic acid in vitamin e deficient rats.
6517909 - Effect of vitamin a deprivation on the mitogenic factor activity in the rat testes.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  116     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-03-27     Completed Date:  1985-03-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1151-7     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chorionic Gonadotropin / classification,  genetics*
Luteinizing Hormone / classification,  genetics
Placenta / metabolism*
RNA / analysis,  metabolism
RNA, Messenger / metabolism*
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/RNA, Messenger; 63231-63-0/RNA; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Early evoked potentials detected from the scalp of man following infraorbital nerve stimulation.
Next Document:  Effect of sodium vanadate on deoxyribonucleic acid and protein syntheses in cultured rat calvariae.