| Absence of detectable chorionic gonadotropin subunit messenger ribonucleic acids in the rat placenta throughout gestation. | |
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MedLine Citation:
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PMID: 2578949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We hypothesized that if a CG similar in structure to other glycoprotein hormones was present in the rat placenta, then mRNAs encoding its subunits would be detectable. To investigate the possible presence of a CG in the rat, we attempted to detect mRNAs encoding the alpha- and CG beta-like subunits in the placentae of timed pregnant rats by sensitive blot hybridization analyses using cloned cDNAs encoding alpha- and beta-subunits of LH derived from rat pituitary mRNA. No subunit-specific mRNAs in placentae of pregnant rats at 4-21 days gestation were detected at either high or low stringencies of hybridization. However, under similar conditions, subunit-specific mRNAs were readily observed in total pituitary RNA of normal as well as ovariectomized rats. Moreover, hybridization with a cDNA for alpha-tubulin, a major component of the cytoskeleton, yielded easily detectable bands in rat placental RNA. In addition, hybridization analysis, under low stringency conditions, of restriction enzyme digests of rat genomic DNA with a rat LH beta-cDNA, which would detect LH beta subunit-like genes, suggests the presence of a single gene that, in fact, encodes the rat LH beta-subunit. We conclude that mRNAs encoding for proteins structurally homologous to rat LH subunits are absent in the rat placenta and that only a single LH beta-like gene is present in the rat. The luteotropic activity associated with the rat placenta must be related to a gonadotropin-like hormone whose structure is dissimilar from that of CG. |
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Authors:
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F E Carr; W W Chin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 116 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1985 Mar |
Date Detail:
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Created Date: 1985-03-27 Completed Date: 1985-03-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1151-7 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chorionic Gonadotropin / classification, genetics* Electrophoresis Female Genes Luteinizing Hormone / classification, genetics Methods Placenta / metabolism* Pregnancy RNA / analysis, metabolism RNA, Messenger / metabolism* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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AM-25532/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/RNA, Messenger; 63231-63-0/RNA; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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