Document Detail


Absence of cytokine response to bacterial challenge in human tubuloepithelial cells.
MedLine Citation:
PMID:  11435742     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In acute bacterial renal infections, which are most frequently caused by Escherichia coli, tubuloepithelial cells are involved with respect to bacterial adherence, invasion and cytotoxicity. In addition, cytokines expressed by tubuloepithelial cells may be relevant for the recruitment of inflammatory cells and tissue damage in bacterial interstitial nephritis. We asked which inflammatory cytokines are produced by primary human tubuloepithelial cells following in vitro exposure to E. coli and found no release of IL-6, IL-8 and TNF-alpha by tubular cells challenged by bacteria. Purified virulence factors (fimbriae, lipopolysaccharide) from E. coli were also without effects on cytokine release by tubular cells. Since lymphocytic infiltration is a characteristic feature in the chronic form of interstitial nephritis, MHC class II expression by tubular cells in response to bacterial coincubation was analyzed. Exposure to both IFN-gamma and E. coli enhanced MHC class II expression on tubuloepithelial cells. In conclusion, tubuloepithelial cells may play an active role in the local defense against bacteria, e.g. by expressing MHC class II molecules. However, in vitro inflammatory cytokines are not induced by E. coli in this cell population.
Authors:
A Leeker; M Ernst; P Lankenau; S Krüger; B Kreft
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney & blood pressure research     Volume:  24     ISSN:  1420-4096     ISO Abbreviation:  Kidney Blood Press. Res.     Publication Date:  2001  
Date Detail:
Created Date:  2001-07-03     Completed Date:  2001-10-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9610505     Medline TA:  Kidney Blood Press Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  105-10     Citation Subset:  IM    
Affiliation:
Medizinische Klinik I, Medizinische Universität zu Lübeck, Deutschland.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis
Cells, Cultured / drug effects,  immunology,  secretion
Culture Media, Conditioned
Cytokines / secretion*
Epithelial Cells / drug effects,  immunology,  secretion
Escherichia coli / physiology*
Escherichia coli Proteins*
Fimbriae, Bacterial / physiology
HLA-DR Antigens / analysis
Hemolysin Proteins / biosynthesis
Humans
Interferon-gamma / pharmacology
Interleukin-1 / pharmacology
Interleukin-6 / secretion
Interleukin-8 / secretion
Kidney Tubules / cytology,  drug effects,  immunology,  secretion*
Lipopolysaccharides / pharmacology
Tumor Necrosis Factor-alpha / secretion
Virulence
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media, Conditioned; 0/Cytokines; 0/Escherichia coli Proteins; 0/HLA-DR Antigens; 0/Hemolysin Proteins; 0/Hlya protein, E coli; 0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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