Document Detail

Absence of T-regulatory cell expression and function in atopic dermatitis skin.
MedLine Citation:
PMID:  16387603     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The role of regulatory T cells has been widely reported in the suppression of T-cell activation. A dysfunction in CD4(+)CD25(+) T-regulatory cell-specific transcription factor FoxP3 leads to immune dysregulation, polyendocrinopathy, enteropathy X-linked syndrome, often associated with atopic dermatitis. Increasing the number and activity of regulatory T cells in affected organs has been suggested as a remedy in various inflammatory diseases, including allergy. OBJECTIVE: To determine the presence and function of regulatory T cells in atopic dermatitis. METHODS: Immunohistochemistry of lesional atopic dermatitis skin and control skin conditions was used to demonstrate regulatory cells and cytokines in situ. The role of effector and regulatory T cells as well as their specific cytokines in apoptosis in human keratinocyte cultures and artificial skin equivalents was investigated. RESULTS: Human T-regulatory type 1 cells, their suppressive cytokines, IL-10 and TGF-beta, as well as receptors for these cytokines were significantly expressed, whereas CD4(+)CD25(+)FoxP3(+) T-regulatory cells were not found in lesional and atopy patch test atopic dermatitis or psoriasis skin. Both subsets of regulatory T cells suppress the allergen-specific activation of T(H)1 and T(H)2 cells. In coculture and artificial skin equivalent experiments, subsets of T-regulatory cells neither induced keratinocyte death nor suppressed apoptosis induced by skin T cells, T(H)1 cells, IFN-gamma, or TNF-alpha. CONCLUSION: A dysregulation of disease-causing effector T cells is observed in atopic dermatitis lesions, in association with an impaired CD4(+)CD25(+)FoxP3(+) T-cell infiltration, despite the expression of type 1 regulatory cells in the dermis.
Johan Verhagen; Mübeccel Akdis; Claudia Traidl-Hoffmann; Peter Schmid-Grendelmeier; Dirkjan Hijnen; Edward F Knol; Heidrun Behrendt; Kurt Blaser; Cezmi A Akdis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  117     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-02     Completed Date:  2006-02-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  176-83     Citation Subset:  AIM; IM    
Swiss Institute of Allergy and Asthma Research, Davos Platz, Switzerland.
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MeSH Terms
Dermatitis, Atopic / immunology*
Interferon-gamma / secretion
Interleukin-10 / analysis,  secretion
Interleukin-4 / secretion
Keratinocytes / cytology
Middle Aged
Receptors, Interleukin / analysis
Receptors, Interleukin-10
Receptors, Transforming Growth Factor beta / analysis
Skin / immunology*
T-Lymphocytes, Regulatory / physiology*
Transforming Growth Factor beta / analysis
Reg. No./Substance:
0/Receptors, Interleukin; 0/Receptors, Interleukin-10; 0/Receptors, Transforming Growth Factor beta; 0/Transforming Growth Factor beta; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

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