| Absence of Mycobacterium avium subsp. paratuberculosis in the microdissected granulomas of Crohn's disease. | |
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MedLine Citation:
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PMID: 15154014 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The etiology of Crohn's disease remains unknown with inflammatory, infectious, and/or genetic causes suspected. Granulomatous inflammation is a characteristic feature of the disorder, resembling the tissue response to mycobacterium. Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent in Johne's disease, a chronic ulcerative intestinal condition in cattle, and has been implicated as a likely candidate. We carefully microdissected the granulomas from the paraffin-embedded resection specimens of 18 patients with well-established Crohn's disease. The DNA obtained was PCR amplified for the IS900 and IS1311 repeat elements of MAP, PCR product size maintained at 101 and 124 base pairs, respectively. Archival tissue from bovine Johne's disease was used as a positive control. MAP-specific DNA, confirmed by sequencing and comparison with prototype strain sequence, was appropriately amplified from the positive control. None of the Crohn's disease cases yielded a positive amplification product, failing to support a role for the organism in the pathogenesis of this illness. |
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Authors:
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Fabien K Baksh; Sydney D Finkelstein; Shashi M Ariyanayagam-Baksh; Patricia A Swalsky; Edwin C Klein; Jean C Dunn |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc Volume: 17 ISSN: 0893-3952 ISO Abbreviation: Mod. Pathol. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-16 Completed Date: 2005-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806605 Medline TA: Mod Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 1289-94 Citation Subset: IM |
Affiliation:
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Department of Pathology, Lancaster General Hospital, PA 17604-3555, USA. fkbaksh@lancastergeneral.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Crohn Disease / microbiology, pathology* DNA, Bacterial / analysis, genetics Electrophoresis, Agar Gel Female Granuloma / microbiology, pathology* Humans Male Microdissection Middle Aged Mycobacterium avium Complex / genetics* Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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