| Absence of microbiota (germ-free conditions) accelerates the atherosclerosis in ApoE-deficient mice fed standard low cholesterol diet. | |
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MedLine Citation:
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PMID: 20379054 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: The aim of our work was to determine the influence of intestinal bacteria on the development of atherosclerotic lesions using apolipoprotein E (ApoE)-deficient knockout mice. METHODS: The experiments were performed on ApoE-/--deficient mouse strain C57BL/6, bred under germ-free (GF) conditions for two generations or under conventional conditions with defined microflora (CV). The mice were fed a standard low cholesterol diet or cholesterol-rich diet for 3-4 months. We studied the development of advanced lesions in the thoracic and abdominal aorta by histological, morphometric and immunohistological methods. RESULTS: Conventionally reared ApoE-/- mice (containing no pathogenic intestinal microbiota) and fed a standard low cholesterol diet in contrast to a high cholesterol diet did not develop atherosclerotic aortic plaques. In contrast, ApoE-/- mice reared under germfree conditions for 2 generations and fed a low cholesterol diet exhibited atherosclerotic plaques in the aorta. Characteristic lipid deposition with foam cells and macrophages was found in their arterial walls. CONCLUSION: In contrast to the absence of atherosclerotic plaques in conventionally reared ApoE-deficient mice, germ-free ApoE-/- mice consuming the same low cholesterol standard diet developed atherosclerotic plaques in the aorta. Differences in atherosclerotic plaques between GF and CV ApoE-/- mice are not so apparent when mice are fed a high cholesterol diet. Our findings thus document the protective effect of microbiota (commensal bacteria) on atherosclerosis development. |
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Authors:
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Renata Stepankova; Zbynek Tonar; Jirina Bartova; Lukas Nedorost; Pavel Rossman; Rudolf Poledne; Martin Schwarzer; Helena Tlaskalova-Hogenova |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-02 |
Journal Detail:
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Title: Journal of atherosclerosis and thrombosis Volume: 17 ISSN: 1880-3873 ISO Abbreviation: J. Atheroscler. Thromb. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9506298 Medline TA: J Atheroscler Thromb Country: Japan |
Other Details:
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Languages: eng Pagination: 796-804 Citation Subset: IM |
Affiliation:
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Department of Immunology and Gnotobiology, Institute of Microbiology, vvi, Czech Academy of Sciences, Praque, Czech Republic. stepankova.renata@seznam.cz |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Abdominal / metabolism, pathology* Aorta, Thoracic / metabolism, pathology* Apolipoproteins E / physiology* Atherosclerosis / etiology, metabolism, prevention & control* Blotting, Western Cholesterol / blood Cholesterol, Dietary / administration & dosage* Disease Models, Animal Female Germ-Free Life Intestinal Mucosa / microbiology Male Metagenome* Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Cholesterol, Dietary; 0/RNA, Messenger; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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