Document Detail

Absence of CIP1/KIP1 hypermethylation in gastric cancer patients from Northern Brazil.
MedLine Citation:
PMID:  20668328     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to investigate the protein expression and methylation pattern of P21(CIP1) and P27(KIP1) genes. PATIENTS AND METHODS: Twenty samples of gastric tumor and non-tumoral tissues of patients from Pará state, Brazil were collected. Methylation patterns were assessed by bisulfite sequencing, and protein expression evaluated with immunohistochemical analysis. RESULTS: None of the analyzed samples showed methylation in both genes. Immunohistochemistry analysis demonstrated mislocalization or absence of expression of P21(CIP1) and P27(KIP1) in 7/20 and 6/20 of the studied samples, respectively. No correlations regarding protein expression and clinicopathological characteristics were observed; down-regulation of expression of P21(CIP1) with low (I-II) tumor stage (p=0.0777), and older age (>50 years old) with negative or mislocalization of P27(KIP1) (p=0.0922) were of borderline the statistical significance. CONCLUSION: Our results suggest that hypermethylation does not contribute to P21(CIP1) and P27(KIP1) silencing in gastric cancer, and that the role of these genes in the gastric tumorigenesis pathways should be studied further in the Pará state population.
Bárbara Do Nascimento Borges; Rommel Mario Rodriguez Burbano; Maria Lúcia Harada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  24     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-07-29     Completed Date:  2010-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  579-82     Citation Subset:  IM    
Laboratório de Biologia Molecular 'Francisco Mauro Salzano', Instituto de Ciências Biológicas, Universidade Federal do Pará, R. Augusto Correa 01, 66075-110 Belém, Pará, Brazil.
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MeSH Terms
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism*
Cyclin-Dependent Kinase Inhibitor p27 / metabolism
DNA Methylation
DNA, Neoplasm / genetics
Gene Expression Regulation, Neoplastic
Intracellular Signaling Peptides and Proteins / genetics,  metabolism*
Middle Aged
Neoplasm Staging
Polymerase Chain Reaction
Promoter Regions, Genetic
Stomach Neoplasms / genetics,  metabolism,  pathology*
Reg. No./Substance:
0/CDKN1A protein, human; 0/CDKN1B protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/DNA, Neoplasm; 0/Intracellular Signaling Peptides and Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27

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