Document Detail


Abscissic acid localization by light microscopic immunohistochemistry in Chenopodium polyspermum L. Effect of water stress.
MedLine Citation:
PMID:  3156177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An indirect immunohistochemical technique was developed using a rabbit anti-abscissic acid (ABA) serum and the soluble peroxidase-antiperoxidase (PAP) complex for the localization of endogenous ABA in the aerial parts of Chenopodium. Terminal bud, axillary bud bearing nodes, and adult leaves were prefixed by a soluble carbodiimide to obtain the coupling of ABA on cellular proteins and postfixed by a conventional mixture of aldehydes. They were then embedded in paraffin or in plastic. Numerous controls were carried out on sections and on a model system to test the validity of the technique. Based on the staining patterns observed along the plant, an apico-basal gradient of ABA was revealed. In the older buds, ABA was mainly concentrated in the quiescent meristematic cells of the apex. Phloem cells of the main axis and chloroplasts of the leaves were specifically labeled. No reaction product was visualized in the parenchyma cells or in the cambial zone. Water stress, which is known to increase ABA content, induced an increase of immunoreactivity within the same compartments. This physiological test validates the stain.
Authors:
B Sotta; L Sossountzov; R Maldiney; I Sabbagh; P Tachon; E Miginiac
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  33     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-04-04     Completed Date:  1985-04-04     Revised Date:  2001-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / analysis*
Cyclohexanecarboxylic Acids / analysis*
Fixatives
Histocytochemistry
Immunochemistry
Immunoenzyme Techniques
Plants, Medicinal / analysis*,  drug effects
Water / pharmacology*
Chemical
Reg. No./Substance:
0/Cyclohexanecarboxylic Acids; 0/Fixatives; 21293-29-8/Abscisic Acid; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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