Document Detail


Abscisic acid has a key role in modulating diverse plant-pathogen interactions.
MedLine Citation:
PMID:  19571312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We isolated an activation-tagged Arabidopsis (Arabidopsis thaliana) line, constitutive disease susceptibility2-1D (cds2-1D), that showed enhanced bacterial growth when challenged with various Pseudomonas syringae strains. Systemic acquired resistance and systemic PATHOGENESIS-RELATED GENE1 induction were also compromised in cds2-1D. The T-DNA insertion adjacent to NINE-CIS-EPOXYCAROTENOID DIOXYGENASE5 (NCED5), one of six genes encoding the abscisic acid (ABA) biosynthetic enzyme NCED, caused a massive increase in transcript level and enhanced ABA levels >2-fold. Overexpression of NCED genes recreated the enhanced disease susceptibility phenotype. NCED2, NCED3, and NCED5 were induced, and ABA accumulated strongly following compatible P. syringae infection. The ABA biosynthetic mutant aba3-1 showed reduced susceptibility to virulent P. syringae, and ABA, whether through exogenous application or endogenous accumulation in response to mild water stress, resulted in increased bacterial growth following challenge with virulent P. syringae, indicating that ABA suppresses resistance to P. syringae. Likewise ABA accumulation also compromised resistance to the biotrophic oomycete Hyaloperonospora arabidopsis, whereas resistance to the fungus Alternaria brassicicola was enhanced in cds2-1D plants and compromised in aba3-1 plants, indicating that ABA promotes resistance to this necrotroph. Comparison of the accumulation of salicylic acid and jasmonic acid in the wild type, cds2-1D, and aba3-1 plants challenged with P. syringae showed that ABA promotes jasmonic acid accumulation and exhibits a complex antagonistic relationship with salicylic acid. Our findings provide genetic evidence that the abiotic stress signal ABA also has profound roles in modulating diverse plant-pathogen interactions mediated at least in part by cross talk with the jasmonic acid and salicylic acid biotic stress signal pathways.
Authors:
Jun Fan; Lionel Hill; Casey Crooks; Peter Doerner; Chris Lamb
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-01
Journal Detail:
Title:  Plant physiology     Volume:  150     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-06     Completed Date:  2009-09-24     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1750-61     Citation Subset:  IM    
Affiliation:
Department of Disease and Stress Biology , John Innes Centre, Norwich NR4 7UH, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / metabolism*
Alleles
Arabidopsis / enzymology,  genetics,  microbiology*
Arabidopsis Proteins / metabolism
Cyclopentanes / metabolism
Dioxygenases
Genes, Dominant
Genes, Plant
Host-Pathogen Interactions*
Immunity, Innate / genetics
Mutation / genetics
Oxygenases / metabolism
Oxylipins / metabolism
Phenotype
Plant Diseases / genetics,  immunology,  microbiology
Plant Proteins
Pseudomonas syringae / physiology
Salicylic Acid / metabolism
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Cyclopentanes; 0/Oxylipins; 0/Plant Proteins; 21293-29-8/Abscisic Acid; 69-72-7/Salicylic Acid; 6RI5N05OWW/jasmonic acid; EC 1.13.-/Oxygenases; EC 1.13.11.-/Dioxygenases; EC 1.13.11.51/9-cis-epoxy-carotenoid dioxygenase
Comments/Corrections

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