Document Detail

Abortive initiation and first bond formation at an activated adenovirus E4 promoter.
MedLine Citation:
PMID:  7592996     Owner:  NLM     Status:  MEDLINE    
Abortive initiation at the adenovirus E4 promoter was studied by following the production of RNA formed from the initiating nucleotides UpA and CTP. Formation of a specific short RNA via a reaction with appropriate alpha-amanitin sensitivity required promoter, activator, and ATP. In the absence of any of these, an alpha-amanitin-resistant reaction led to lower levels of a product of unknown origin. The alpha-amanitin-sensitive reaction required open promoter complexes, as assayed directly by permanganate probing. This reaction was not blocked by the inhibition of polymerase C-terminal domain kinase activity or by the lack of DNA supercoiling. Thus, formation of the initial bond of the mRNA appears to require activator and ATP to open the DNA but not phosphorylation of the polymerase C-terminal domain. In addition, the abortive initiation reaction was strongly suppressed when all elongation substrates were present, suggesting that cycling to produce high amounts of abortive product is strongly disfavored during productive initiation at this promoter.
Y Jiang; M Yan; J D Gralla
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-26     Completed Date:  1995-12-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27332-8     Citation Subset:  IM    
Department of Chemistry and Biochemistry, University of California, Los Angeles 90095-1569, USA.
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MeSH Terms
Adenosine Triphosphate / metabolism
Adenoviridae / genetics*,  metabolism*
Amanitins / pharmacology
DNA, Superhelical / chemistry,  metabolism
DNA, Viral / chemistry,  metabolism
Gene Expression Regulation
Hela Cells
Promoter Regions, Genetic*
Protein Kinases / metabolism
RNA Polymerase II / metabolism
RNA, Messenger / biosynthesis,  chemistry
RNA, Viral / biosynthesis*,  chemistry
Grant Support
Reg. No./Substance:
0/Amanitins; 0/DNA, Superhelical; 0/DNA, Viral; 0/RNA, Messenger; 0/RNA, Viral; 56-65-5/Adenosine Triphosphate; EC 2.7.-/Protein Kinases; EC 2.7.1.-/carboxy-terminal domain kinase; EC 2.7.7.-/RNA Polymerase II

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