| Abolition of biofilm formation in urinary tract Escherichia coli and Klebsiella isolates by metal interference through competition for fur. | |
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MedLine Citation:
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PMID: 20418434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterial biofilms are associated with a large number of persistent and chronic infections. Biofilm-dwelling bacteria are particularly resistant to antibiotics and immune defenses, which makes it hard if not impossible to eradicate biofilm-associated infections. In the urinary tract, free iron is strictly limited but is critical for bacterial growth. Biofilm-associated Escherichia coli cells are particularly desperate for iron. An attractive way of inhibiting biofilm formation is to fool the bacterial regulatory system for iron uptake. Here, we demonstrate that biofilm formation can be impaired by the addition of divalent metal ions, such as Zn(II) and Co(II), which inhibit iron uptake by virtue of their higher-than-iron affinity for the master controller protein of iron uptake, Fur. Reduced biofilm formation of urinary tract-infectious E. coli strains in the presence of Zn(II) was observed in microtiter plates and flow chambers as well as on urinary catheters. These results further support that iron uptake is indeed crucial for biofilm formation, and thereby, targeting these uptake systems might be an effective way to eradicate biofilms caused by infectious strains. |
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Authors:
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Viktoria Hancock; Malin Dahl; Per Klemm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-23 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-07 Completed Date: 2010-09-28 Revised Date: 2011-03-03 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3836-41 Citation Subset: IM |
Affiliation:
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Microbial Adhesion Group, DTU Food, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. pkl@cbs.dtu.dk. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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metabolism Bacterial Proteins / antagonists & inhibitors* Biofilms / drug effects, growth & development* Cations, Divalent / metabolism Colony Count, Microbial Escherichia coli / drug effects*, physiology Iron / antagonists & inhibitors* Klebsiella / drug effects*, physiology Metals / metabolism* Repressor Proteins / antagonists & inhibitors* Urinary Tract / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Cations, Divalent; 0/Metals; 0/Repressor Proteins; 0/ferric uptake regulating proteins, bacterial; 7439-89-6/Iron |
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