Document Detail

Abnormalities of stromal structure in the bullous keratopathy cornea identified by second harmonic generation imaging microscopy.
MedLine Citation:
PMID:  22736613     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To identify structural alterations in collagen lamellae and the transdifferentiation of keratocytes into myofibroblasts in the corneal stroma of bullous keratopathy (BK) patients and to examine the relation of such changes to the duration of stromal edema or the underlying cause of BK.
METHODS: Six normal human corneas and 16 BK corneas were subjected to second harmonic generation (SHG) imaging microscopy to allow three-dimensional (3-D) reconstruction of collagen lamellae. Expression of α-smooth muscle actin (αSMA) was examined by immunofluorescence analysis and conventional laser confocal microscopy.
RESULTS: Collagen lamellae were interwoven at the anterior stroma and uniformly aligned at the posterior stroma, whereas αSMA was not detected throughout the entire stroma of the normal cornea. Nine (56%) and 7 (44%) of the 16 BK corneas showed abnormal collagen structure at the anterior and posterior stroma, respectively. Expression of αSMA was detected in the anterior or posterior stroma of 7 (44%) and 6 (38%) of the 16 BK corneas, respectively. Disorganization of collagen lamellae and myofibroblastic transdifferentiation were detected only in corneas with a duration of stromal edema of at least 12 months. Corneas with BK as a result of birth injury showed abnormal collagen structure at the posterior stroma, whereas those with BK resulting from laser iridotomy did not.
CONCLUSIONS: Changes in the structure of the entire stroma were detected in BK corneas with a duration of stromal edema of at least 12 months, suggesting that such changes may be progressive. In addition, the underlying cause of BK may influence structural changes at the posterior stroma.
Naoyuki Morishige; Norihiro Yamada; Xu Zhang; Yukiko Morita; Naoyuki Yamada; Kazuhiro Kimura; Atsushi Takahara; Koh-Hei Sonoda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-10-15     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4998-5003     Citation Subset:  IM    
Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
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MeSH Terms
Actins / ultrastructure*
Aged, 80 and over
Cell Transdifferentiation / physiology
Collagen / ultrastructure
Corneal Diseases / pathology*
Corneal Stroma / abnormalities*
Imaging, Three-Dimensional
Keratinocytes / cytology
Middle Aged
Myofibroblasts / cytology
Reg. No./Substance:
0/ACTA2 protein, human; 0/Actins; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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