Document Detail

Abnormal uterine stromal and glandular function associated with maternal reproductive defects in Hoxa-11 null mice.
MedLine Citation:
PMID:  9160706     Owner:  NLM     Status:  MEDLINE    
Here we describe in detail both the expression of Hoxa-11 in the wild-type mouse uterus and the defects resulting in maternal reproductive failure of Hoxa-11 null female mice. The Hoxa-11 gene is expressed at peak levels in uterine stromal cells during metestrus. Hoxa-11 transcripts were induced beginning on Day 2 of gestation in the stromal cells underlying the uterine epithelium and appeared in the secondary decidual zone between Days 6 and 8 of gestation. At early gestational stages, stromal, decidual, and glandular cell development were deficient in Hoxa-11 null uteri in comparison to wild-type as assessed by histology and immunohistochemical localization of the decidual cell marker epitope, stage-specific embryonic antigen-3 (SSEA-3). Both steroid-induced uterine stromal and glandular cell proliferation as well as oil-induced stromal decidualization after induction of pseudopregnancy were deficient in mutant uteri. Moreover, both Western blotting and immunohistochemistry demonstrated that the burst of glandular leukemia inhibitory factor (LIF) found in normal pregnant uteri at Day 4.5 of gestation was absent in Hoxa-11-deficient uteri. The LIF burst was also not observed in the uteri of bilaterally ovariectomized, hormonally stimulated Hoxa-11 mutants. These results demonstrate that the Hoxa-11 gene is required for normal uterine stromal cell and glandular differentiation during pregnancy, as is the presence of the steroid-induced glandular LIF burst initiating embryo implantation.
R L Gendron; H Paradis; H M Hsieh-Li; D W Lee; S S Potter; E Markoff
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  56     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-07-28     Completed Date:  1997-07-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1097-105     Citation Subset:  IM    
Department of Pediatrics, The Children's Hospital Research Foundation, Cincinnati, Ohio 45229-3039, USA.
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MeSH Terms
Antigens, Tumor-Associated, Carbohydrate
Decidua / pathology,  physiopathology
Genes, Homeobox*
Glycosphingolipids / metabolism
Growth Inhibitors / biosynthesis
Homeodomain Proteins / genetics*
In Situ Hybridization
Leukemia Inhibitory Factor
Lymphokines / biosynthesis
Mice, Knockout
Ovary / physiology
Pseudopregnancy / genetics,  physiopathology
RNA, Messenger / genetics,  metabolism
Reproduction / genetics*,  physiology
Stage-Specific Embryonic Antigens
Uterus / abnormalities*,  physiopathology*
Grant Support
Reg. No./Substance:
0/Antigens, Tumor-Associated, Carbohydrate; 0/Glycosphingolipids; 0/Growth Inhibitors; 0/Homeodomain Proteins; 0/Hoxa11 protein, mouse; 0/Interleukin-6; 0/Leukemia Inhibitory Factor; 0/Lif protein, mouse; 0/Lymphokines; 0/RNA, Messenger; 0/Stage-Specific Embryonic Antigens; 0/stage-specific embryonic antigen-3

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