Document Detail

Abnormal structural luteolysis in ovaries of the senescence accelerated mouse (SAM): expression of Fas ligand/Fas-mediated apoptosis signaling molecules in luteal cells.
MedLine Citation:
PMID:  14967896     Owner:  NLM     Status:  MEDLINE    
Senescence accelerated mouse-prone (SAMP) mice with a shortened life span show accelerated changes in many of the signs of aging and a shorter reproductive life span than SAM-resistant (SAMR) controls. We previously showed that functional regression (progesterone dissimilation) occurs in abnormally accumulated luteal bodies (aaLBs) of SAMP mice, but structural regression of luteal cells in aaLB is inhibited. A deficiency of luteal cell apoptosis causes the abnormal accumulation of LBs in SAMP ovaries. In the present study, to show the abnormality of Fas ligand (FasL)/Fas-mediated apoptosis signal transducing factors in the aaLBs of the SAMP ovaries, we assessed the changes in the expression of FasL, Fas, caspase-8 and caspase-3 mRNAs by reverse transcription-polymerase chain reaction, and in the expression and localization of FasL, Fas and activated caspase-3 proteins by Western blotting and immunohistochemistry, respectively, during the estrus cycle/luteolysis. These mRNAs and proteins were expressed in normal LBs of both SAMP and SAMR ovaries, but not at all or only in trace amounts in aaLBs of SAMP, indicating that structural regression is inhibited by blockage of the expression of these transducing factors in luteal cells of aaLBs in SAMP mice.
Minako Kiso; Noboru Manabe; Kohji Komatsu; Munetake Shimabe; Hajime Miyamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  49     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-02-17     Completed Date:  2004-09-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  457-63     Citation Subset:  IM    
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Japan.
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MeSH Terms
Antigens, CD95 / biosynthesis*
Blotting, Western
Caspase 3
Caspase 8
Caspases / metabolism
DNA, Single-Stranded / metabolism
Enzyme Activation
Estrous Cycle
Fas Ligand Protein
Luteal Cells / metabolism*
Membrane Glycoproteins / biosynthesis*
Ovary / physiology*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Reg. No./Substance:
0/Antigens, CD95; 0/DNA, Single-Stranded; 0/Fas Ligand Protein; 0/Fasl protein, mouse; 0/Membrane Glycoproteins; 0/RNA, Messenger; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Casp8 protein, mouse; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases

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