| Abnormal serum concentrations of proteins in Parkinson's disease. | |
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MedLine Citation:
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PMID: 19723509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Blood serum was used to identify protein biomarkers for diagnosis of Parkinson's disease (PD) using analytically validated quantitative 2D-gel electrophoresis, and single variable and multivariate statistics. Using banked samples from a first medical center, we identified 57 specific protein spot biomarkers with disease-specific abnormal levels in serum of patients with PD, Alzheimer's disease, amyotrophic lateral sclerosis and similar neurodegenerative conditions (337 samples), when compared to age-matched normal controls (132 samples). To further assess their clinical usefulness in Parkinson's disease, we obtained prospective newly drawn blood serum samples from a second (56 PD, 30 controls) and third (6 PD, 48 controls) medical center. The protein concentrations of the 57 biomarkers were assessed by 2D-gel electrophoresis. Stepwise linear discriminant analysis selected a combination of 21 of the 57 as optimal to distinguish PD patients from controls. When applied to the samples from the second site, the 21 proteins had sensitivity of 93.3% (52 of 56 PD correctly classified), specificity of 92.9% (28 of 30 controls correctly classified); 15 of 15 patients with mild, 28 of 30 with moderate to severe symptoms, and all of the 6 PD patients from the third site were correctly classified. Eleven of the 21 proteins showed statistically significant abnormal concentrations in patients with mild symptoms, and 14 in patients with moderate-severe symptoms. The protein identities reflect the heterogeneity of Parkinson's disease, and thus may provide the capability of monitoring the blood for a diverse range of PD pathophysiological mechanisms: cellular degeneration, oxidative stress, inflammation, and transport. |
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Authors:
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Ira L Goldknopf; Jennifer K Bryson; Irina Strelets; Silvia Quintero; Essam A Sheta; Miguel Mosqueda; Helen R Park; Stanley H Appel; Holly Shill; Marwan Sabbagh; Bruce Chase; Eric Kaldjian; Katerina Markopoulou |
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Publication Detail:
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Type: Journal Article Date: 2009-08-31 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 389 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-09-29 Completed Date: 2009-10-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 321-7 Citation Subset: IM |
Affiliation:
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Power3 Medical Products, Inc., The Woodlands, TX 77381, USA. igoldknopf@power3medical.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Biological Markers / blood Blood Proteins / analysis* Female Humans Male Middle Aged Parkinson Disease / blood* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Blood Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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