Document Detail


Abnormal serum concentrations of proteins in Parkinson's disease.
MedLine Citation:
PMID:  19723509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blood serum was used to identify protein biomarkers for diagnosis of Parkinson's disease (PD) using analytically validated quantitative 2D-gel electrophoresis, and single variable and multivariate statistics. Using banked samples from a first medical center, we identified 57 specific protein spot biomarkers with disease-specific abnormal levels in serum of patients with PD, Alzheimer's disease, amyotrophic lateral sclerosis and similar neurodegenerative conditions (337 samples), when compared to age-matched normal controls (132 samples). To further assess their clinical usefulness in Parkinson's disease, we obtained prospective newly drawn blood serum samples from a second (56 PD, 30 controls) and third (6 PD, 48 controls) medical center. The protein concentrations of the 57 biomarkers were assessed by 2D-gel electrophoresis. Stepwise linear discriminant analysis selected a combination of 21 of the 57 as optimal to distinguish PD patients from controls. When applied to the samples from the second site, the 21 proteins had sensitivity of 93.3% (52 of 56 PD correctly classified), specificity of 92.9% (28 of 30 controls correctly classified); 15 of 15 patients with mild, 28 of 30 with moderate to severe symptoms, and all of the 6 PD patients from the third site were correctly classified. Eleven of the 21 proteins showed statistically significant abnormal concentrations in patients with mild symptoms, and 14 in patients with moderate-severe symptoms. The protein identities reflect the heterogeneity of Parkinson's disease, and thus may provide the capability of monitoring the blood for a diverse range of PD pathophysiological mechanisms: cellular degeneration, oxidative stress, inflammation, and transport.
Authors:
Ira L Goldknopf; Jennifer K Bryson; Irina Strelets; Silvia Quintero; Essam A Sheta; Miguel Mosqueda; Helen R Park; Stanley H Appel; Holly Shill; Marwan Sabbagh; Bruce Chase; Eric Kaldjian; Katerina Markopoulou
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Publication Detail:
Type:  Journal Article     Date:  2009-08-31
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  389     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-29     Completed Date:  2009-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  321-7     Citation Subset:  IM    
Affiliation:
Power3 Medical Products, Inc., The Woodlands, TX 77381, USA. igoldknopf@power3medical.com
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Biological Markers / blood
Blood Proteins / analysis*
Female
Humans
Male
Middle Aged
Parkinson Disease / blood*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Blood Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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