Document Detail


Abnormal proteins enhance stress-induced cell death.
MedLine Citation:
PMID:  9473497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of abnormal proteins on cell viability was studied using artificially cleaved polypeptides. Escherichia coli methionyl-tRNA synthetase (MetRS) consists of two distinct domains and its activity is essential for cell viability. The polypeptide chain was split by linker insertion and expressed as two fragments. Two pairs of polypeptides, one split within the N-terminal domain and another at the junction of the two domains retained aminoacylation activity. The in vitro activities of these split mutants were enhanced by the presence of chaperonin, GroESL. However, cells containing these split polypeptides became sensitive to conditions that induce GroESL. The results of this work suggest that an abnormally generated protein can cause cell death under stressful conditions.
Authors:
S J Kim; S Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  243     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-16     Completed Date:  1998-03-16     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  153-7     Citation Subset:  IM    
Affiliation:
Department of Biology, Sung Kyun Kwan University, Kyunggido, Korea.
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MeSH Terms
Descriptor/Qualifier:
Anti-Infective Agents / pharmacology
Bacterial Proteins / chemistry,  genetics,  pharmacology*
Chaperonins / chemistry,  genetics,  pharmacology
Enzyme Stability
Escherichia coli / drug effects*,  enzymology,  growth & development
Fluoroquinolones*
Methionine-tRNA Ligase / chemistry,  genetics,  pharmacology
Models, Molecular
Mutation
Ofloxacin / pharmacology
Peptide Fragments / chemistry,  genetics,  pharmacology
Protein Conformation
Quinolones / pharmacology
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Bacterial Proteins; 0/Fluoroquinolones; 0/GroESL protein, Bacteria; 0/Peptide Fragments; 0/Quinolones; 82419-36-1/Ofloxacin; EC 3.6.1.-/Chaperonins; EC 6.1.1.10/Methionine-tRNA Ligase; Q90AGA787L/sparfloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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